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Applied and Environmental Microbiology, February 2006, p. 1198-1206, Vol. 72, No. 2
0099-2240/06/$08.00+0     doi:10.1128/AEM.72.2.1198-1206.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Coexistence of Burkholderia, Cupriavidus, and Rhizobium sp. Nodule Bacteria on two Mimosa spp. in Costa Rica

Craig F. Barrett{dagger} and Matthew A. Parker*

Department of Biological Sciences, State University of New York, Binghamton, New York 13902

Received 23 September 2005/ Accepted 20 November 2005

rRNA gene sequencing and PCR assays indicated that 215 isolates of root nodule bacteria from two Mimosa species at three sites in Costa Rica belonged to the genera Burkholderia, Cupriavidus, and Rhizobium. This is the first report of Cupriavidus sp. nodule symbionts for Mimosa populations within their native geographic range in the neotropics. Burkholderia spp. predominated among samples from Mimosa pigra (86% of isolates), while there was a more even distribution of Cupriavidus, Burkholderia, and Rhizobium spp. on Mimosa pudica (38, 37, and 25% of isolates, respectively). All Cupriavidus and Burkholderia genotypes tested formed root nodules and fixed nitrogen on both M. pigra and M. pudica, and sequencing of rRNA genes in strains reisolated from nodules verified identity with inoculant strains. Inoculation tests further indicated that both Cupriavidus and Burkholderia spp. resulted in significantly higher plant growth and nodule nitrogenase activity (as measured by acetylene reduction assays) relative to plant performance with strains of Rhizobium. Given the prevalence of Burkholderia and Cupriavidus spp. on these Mimosa legumes and the widespread distribution of these plants both within and outside the neotropics, it is likely that both ß-proteobacterial genera are more ubiquitous as root nodule symbionts than previously believed.

* Corresponding author. Mailing address: Department of Biological Sciences, State University of New York, Binghamton, NY 13902. Phone: (607) 777-6283. Fax: (607) 777-6521. E-mail: mparker{at}binghamton.edu.

{dagger} Present address: Ohio State University Herbarium, Museum of Biological Diversity, Columbus, OH 43212.

Applied and Environmental Microbiology, February 2006, p. 1198-1206, Vol. 72, No. 2
0099-2240/06/$08.00+0     doi:10.1128/AEM.72.2.1198-1206.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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