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Applied and Environmental Microbiology, February 2006, p. 1267-1269, Vol. 72, No. 2
0099-2240/06/$08.00+0     doi:10.1128/AEM.72.2.1267-1269.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

RNA-Mediated Gene Silencing in Monokaryons and Dikaryons of Schizophyllum commune

Jan F. de Jong, Heine J. Deelstra, Han A. B. Wösten, and Luis G. Lugones*

Microbiology, Institute of Biomembranes, University of Utrecht, Padualaan 8, 3584 CH Utrecht, The Netherlands

Received 6 July 2005/ Accepted 29 November 2005

Disruption of genes by homologous recombination occurs at a low frequency in the basidiomycete Schizophyllum commune. For instance, the SC3 and SC15 genes were inactivated at frequencies of 1 and 5%, respectively. As an alternative to disruption, we used gene silencing through the introduction of a hairpin construct. The SC15 gene, which encodes an abundantly secreted structural protein, was silenced at a frequency of 80% in monokaryons of S. commune after introduction of a hairpin construct of the gene. Silencing also occurred in dikaryons in which one of the partners was not a silenced strain. The silencing mechanism resembles RNAi in other filamentous fungi and is a powerful tool for the functional analysis of genes expressed in monokaryons or dikaryons.

* Corresponding author. Mailing address: Microbiology, Institute of Biomembranes, University of Utrecht, Padualaan 8, NL-3584 CH Utrecht, The Netherlands. Phone: (31) 30 2533016. Fax: (31) 30 2513655. E-mail: l.g.lugones{at}bio.uu.nl.

Applied and Environmental Microbiology, February 2006, p. 1267-1269, Vol. 72, No. 2
0099-2240/06/$08.00+0     doi:10.1128/AEM.72.2.1267-1269.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

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  • Janus, D., Hoff, B., Hofmann, E., Kuck, U. (2007). An Efficient Fungal RNA-Silencing System Using the DsRed Reporter Gene. Appl. Environ. Microbiol. 73: 962-970 [Abstract] [Full Text]  


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