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Applied and Environmental Microbiology, February 2006, p. 1429-1436, Vol. 72, No. 2
0099-2240/06/$08.00+0     doi:10.1128/AEM.72.2.1429-1436.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Application of Streptococcus uberis Multilocus Sequence Typing: Analysis of the Population Structure Detected among Environmental and Bovine Isolates from New Zealand and the United Kingdom

Gillian D. Pullinger,1 Mario López-Benavides,2 Tracey J. Coffey,1 John H. Williamson,2 Ray T. Cursons,3 Emma Summers,2 Jane Lacy-Hulbert,2 Martin C. Maiden,4 and James A. Leigh1*

Institute for Animal Health, Compton, Newbury, Berkshire RG20 7NN, United Kingdom,1 Dexcel, Ltd., Hamilton, New Zealand,2 University of Waikato, Waikato, New Zealand,3 The Peter Medawar Building for Pathogen Research, University of Oxford, South Parks Rd., Oxford OX1 3SY, United Kingdom4

Received 30 September 2005/ Accepted 29 November 2005

We recently developed a multilocus sequence typing (MLST) scheme to differentiate S. uberis isolates and facilitate an understanding of the population biology of this pathogen. The scheme was initially used to study a collection of 160 bovine milk isolates from the United Kingdom and showed that the majority of isolates were from one clonal complex (designated the ST-5 complex). Here we describe the MLST analysis of a collection of New Zealand isolates. These were obtained from diverse sources, including bovine milk, other bovine anatomical sites, and environmental sources. The complete allelic profiles of 253 isolates were determined. The collection was highly diverse and included 131 different sequence types (STs). The New Zealand and United Kingdom populations were distinct, since none of the 131 STs were represented within the previously studied collection of 160 United Kingdom S. uberis isolates. However, seven of the STs were members of the ST-5 clonal complex, the major complex within the United Kingdom collection. Two new clonal complexes were identified: ST-143 and ST-86. All three major complexes were isolated from milk, other bovine sites, and the environment. Carriage of the hasA gene, which is necessary for capsule formation, correlated with clonal complex and isolation from clinical cases of mastitis.

* Corresponding author. Present address: Nuffield Department of Clinical Laboratory Sciences, John Radcliffe Hospital, University of Oxford, Oxford OX3 9DU, United Kingdom. Phone: 44 1865 221226. Fax: 44 1865 764192. E-mail: james.leigh{at}ndcls.ox.ac.uk.

Applied and Environmental Microbiology, February 2006, p. 1429-1436, Vol. 72, No. 2
0099-2240/06/$08.00+0     doi:10.1128/AEM.72.2.1429-1436.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Pryor, S. M., Cursons, R. T., Williamson, J. H., Lacy-Hulbert, S. J. (2009). Experimentally induced intramammary infection with multiple strains of Streptococcus uberis. J DAIRY SCI 92: 5467-5475 [Abstract] [Full Text]  
  • Rato, M. G., Bexiga, R., Nunes, S. F., Cavaco, L. M., Vilela, C. L., Santos-Sanches, I. (2008). Molecular Epidemiology and Population Structure of Bovine Streptococcus uberis. J DAIRY SCI 91: 4542-4551 [Abstract] [Full Text]  
  • Tomita, T., Meehan, B., Wongkattiya, N., Malmo, J., Pullinger, G., Leigh, J., Deighton, M. (2008). Identification of Streptococcus uberis Multilocus Sequence Types Highly Associated with Mastitis. Appl. Environ. Microbiol. 74: 114-124 [Abstract] [Full Text]  
  • Lopez-Benavides, M. G., Williamson, J. H., Pullinger, G. D., Lacy-Hulbert, S. J., Cursons, R. T., Leigh, J. A. (2007). Field Observations on the Variation of Streptococcus uberis Populations in a Pasture-Based Dairy Farm. J DAIRY SCI 90: 5558-5566 [Abstract] [Full Text]  


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