This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Kim, J.-E. Articles by Lee, Y.-W. Search for Related Content PubMed PubMed Citation Articles by Kim, J.-E. Articles by Lee, Y.-W. Agricola Articles by Kim, J.-E. Articles by Lee, Y.-W.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, February 2006, p. 1645-1652, Vol. 72, No. 2
0099-2240/06/$08.00+0     doi:10.1128/AEM.72.2.1645-1652.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

GIP2, a Putative Transcription Factor That Regulates the Aurofusarin Biosynthetic Gene Cluster in Gibberella zeae

Jung-Eun Kim,¹ Jianming Jin,¹ Hun Kim,¹ Jin-Cheol Kim,² Sung-Hwan Yun,³ and Yin-Won Lee^1*

School of Agricultural Biotechnology and Center for Agricultural Biomaterials, Seoul National University, Seoul 151-921,¹ Biological Function Research Team, Korea Research Institute of Chemical Technology, Daejon 305-606,² Division of Life Sciences, Soonchunhyang University, Asan 336-745, Korea³

Received 17 August 2005/ Accepted 9 December 2005

Gibberella zeae (anamorph: Fusarium graminearum) is an important pathogen of maize, wheat, and rice. Colonies of G. zeae produce yellow-to-tan mycelia with the white-to-carmine red margins. In this study, we focused on nine putative open reading frames (ORFs) closely linked to PKS12 and GIP1, which are required for aurofusarin biosynthesis in G. zeae. Among them is an ORF designated GIP2 (for Gibberella zeae pigment gene 2), which encodes a putative protein of 398 amino acids that carries a Zn(II)₂Cys₆ binuclear cluster DNA-binding domain commonly found in transcription factors of yeasts and filamentous fungi. Targeted gene deletion and complementation analyses confirmed that GIP2 is required for aurofusarin biosynthesis. Expression of GIP2 in carrot medium correlated with aurofusarin production by G. zeae and was restricted to vegetative mycelia. Inactivation of the 10 contiguous genes in the GIP2 strain delineates an aurofusarin biosynthetic gene cluster. Overexpression of GIP2 in both the GIP2 and the wild-type strains increases aurofusarin production and reduces mycelial growth. Thus, GIP2 is a putative positive regulator of the aurofusarin biosynthetic gene cluster, and aurofusarin production is negatively correlated with vegetative growth by G. zeae.

---

* Corresponding author. Mailing address: School of Agricultural Biotechnology and Center for Agricultural Biomaterials, Seoul National University, Seoul 151-921, Korea. Phone: 82 2 880 4671. Fax: 82 2 873 2317. E-mail: lee2443{at}snu.ac.kr.

---

Applied and Environmental Microbiology, February 2006, p. 1645-1652, Vol. 72, No. 2
0099-2240/06/$08.00+0     doi:10.1128/AEM.72.2.1645-1652.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

This article has been cited by other articles:

• Ding, S., Mehrabi, R., Koten, C., Kang, Z., Wei, Y., Seong, K., Kistler, H. C., Xu, J.-R. (2009). Transducin Beta-Like Gene FTL1 Is Essential for Pathogenesis in Fusarium graminearum. Eukaryot Cell 8: 867-876 [Abstract] [Full Text]  
• Lee, S.-H., Lee, J., Lee, S., Park, E.-H., Kim, K.-W., Kim, M.-D., Yun, S.-H., Lee, Y.-W. (2009). GzSNF1 Is Required for Normal Sexual and Asexual Development in the Ascomycete Gibberella zeae. Eukaryot Cell 8: 116-127 [Abstract] [Full Text]