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Applied and Environmental Microbiology, February 2006, p. 1672-1676, Vol. 72, No. 2
0099-2240/06/$08.00+0     doi:10.1128/AEM.72.2.1672-1676.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

mRNA-Based Parallel Detection of Active Methanotroph Populations by Use of a Diagnostic Microarray

Levente Bodrossy,1* Nancy Stralis-Pavese,1 Marianne Konrad-Köszler,1 Alexandra Weilharter,1 Thomas G. Reichenauer,2 David Schöfer,2 and Angela Sessitsch1

Department of Bioresources,1 Department of Environmental Research, ARC Seibersdorf Research GmbH, A-2444 Seibersdorf, Austria2

Received 17 August 2005/ Accepted 21 November 2005

A method was developed for the mRNA-based application of microbial diagnostic microarrays to detect active microbial populations. DNA- and mRNA-based analyses of environmental samples were compared and confirmed via quantitative PCR. Results indicated that mRNA-based microarray analyses may provide additional information on the composition and functioning of microbial communities.

* Corresponding author. Mailing address: Department of Bioresources, ARC Seibersdorf Research GmbH, A-2444 Seibersdorf, Austria. Phone: 43 0 50550 3548. Fax: 43 0 50550 3444. E-mail: levente.bodrossy{at}arcs.ac.at.

Applied and Environmental Microbiology, February 2006, p. 1672-1676, Vol. 72, No. 2
0099-2240/06/$08.00+0     doi:10.1128/AEM.72.2.1672-1676.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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