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Applied and Environmental Microbiology, February 2006, p. 1677-1679, Vol. 72, No. 2
0099-2240/06/$08.00+0     doi:10.1128/AEM.72.2.1677-1679.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

A Bacillus megaterium Plasmid System for the Production, Export, and One-Step Purification of Affinity-Tagged Heterologous Levansucrase from Growth Medium{dagger}

Marco Malten,1,{ddagger} Rebekka Biedendieck,1,{ddagger} Martin Gamer,1 Ann-Christin Drews,1 Simon Stammen,1 Klaus Buchholz,2 Lubbert Dijkhuizen,3 and Dieter Jahn1*

Institute of Microbiology, Technical University Braunschweig, Spielmannstrasse 7, D-38106 Braunschweig, Germany,1 Institute for Technical Chemistry, Technology of Carbohydrates, Hans-Sommer-Strasse 10, D-38106 Braunschweig, Germany,2 Microbial Physiology and Centre for Carbohydrate Bioprocessing (CCB, TNO Quality of Life-University of Groningen), Groningen Biomolecular Sciences and Biotechnology Institute (GBB), University of Groningen, Kerklaan 30, NL-9751 NN Haren, The Netherlands3

Received 30 September 2005/ Accepted 4 November 2005

A multiple vector system for the production and export of recombinant affinity-tagged proteins in Bacillus megaterium was developed. Up to 1 mg/liter of a His6-tagged or Strep-tagged Lactobacillus reuteri levansucrase was directed into the growth medium, using the B. megaterium esterase LipA signal peptide, and recovered by one-step affinity chromatography.

* Corresponding author. Mailing address: Institute of Microbiology, Technical University Braunschweig, Spielmannstrasse 7, D-38106 Braunschweig, Germany. Phone: 49 (0) 531-391-5801. Fax: 49 (0) 531-391-5854. E-mail: d.jahn{at}tu-bs.de.

{dagger} Supplemental material for this article may be found at http://aem.asm.org/.

{ddagger} M. Malten and R. Biedendieck contributed equally to this work.

Applied and Environmental Microbiology, February 2006, p. 1677-1679, Vol. 72, No. 2
0099-2240/06/$08.00+0     doi:10.1128/AEM.72.2.1677-1679.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.





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Copyright © 2006 by the American Society for Microbiology. All rights reserved.