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Applied and Environmental Microbiology, February 2006, p. 1692-1695, Vol. 72, No. 2
0099-2240/06/$08.00+0     doi:10.1128/AEM.72.2.1692-1695.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Measurement of Respiration Rates of Methylobacterium extorquens AM1 Cultures by Use of a Phosphorescence-Based Sensor

Tim J. Strovas,1,{dagger} Joe M. Dragavon,2,{dagger} Tyler J. Hankins,2 James B. Callis,2 Lloyd W. Burgess,2 and Mary E. Lidstrom3,4*

Department of Bioengineering,1 Department of Chemistry,2 Department of Chemical Engineering,3 Department of Microbiology, Microscale Life Sciences Center, University of Washington, Seattle, Washington 98195-21804

Received 19 July 2005/ Accepted 24 November 2005

Respiration rates of bacterial cultures can be a powerful tool in gauging the effects of genetic manipulation and environmental changes affecting overall metabolism. We present an optical method for measuring respiration rates using a robust phosphorescence lifetime-based sensor and off-the-shelf technology. This method was tested with the facultative methylotroph Methylobacterium extorquens AM1 to demonstrate subtle mutant phenotypes.

* Corresponding author. Mailing address: Department of Chemical Engineering, University of Washington, Box 352125, Seattle, WA 98195. Phone: (206) 616-5282. Fax: (206) 616-5721. E-mail: lidstrom{at}u.washington.edu.

{dagger} T.J.S. and J.M.D. contributed equally to this study.

Applied and Environmental Microbiology, February 2006, p. 1692-1695, Vol. 72, No. 2
0099-2240/06/$08.00+0     doi:10.1128/AEM.72.2.1692-1695.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Dragavon, J., Molter, T., Young, C., Strovas, T., McQuaide, S., Holl, M., Zhang, M., Cookson, B., Jen, A., Lidstrom, M., Meldrum, D., Burgess, L. (2008). A cellular isolation system for real-time single-cell oxygen consumption monitoring. J R Soc Interface 5: S151-S159 [Abstract] [Full Text]  


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