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Applied and Environmental Microbiology, February 2006, p. 1705-1707, Vol. 72, No. 2
0099-2240/06/$08.00+0     doi:10.1128/AEM.72.2.1705-1707.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Isolation of Rickettsia felis in the Mosquito Cell Line C6/36

Maurício C. Horta,1 Marcelo B. Labruna,1* Edison L. Durigon,2 and Teresinha T. S. Schumaker3

Faculty of Veterinary Medicine, University of Sao Paulo, São Paulo, SP 05508-270, Brazil,1 Department of Microbiology, University of Sao Paulo, São Paulo, SP 05508-900, Brazil,2 Department of Parasitology, University of Sao Paulo, São Paulo, SP 05508-900, Brazil3

Received 15 September 2005/ Accepted 28 November 2005

We report the isolation and establishment of Rickettsia felis in the C6/36 cell line. Rickettsial growth was intense, always with 90 to 100% of cells being infected after few weeks. The rickettsial isolate was confirmed by testing infected cells by PCR and sequencing fragments of three major Rickettsia genes (gltA, ompB, and the 17-kDa protein gene).

* Corresponding author. Mailing address: Laboratório de Doenças Parasitárias, Departamento de Medicina Veterinária Preventiva e Saúde Animal, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo, SP 05508-270, Brazil. Phone: 55-11-3091 1394. Fax: 55-11-3091 7928. E-mail: labruna{at}usp.br.

Applied and Environmental Microbiology, February 2006, p. 1705-1707, Vol. 72, No. 2
0099-2240/06/$08.00+0     doi:10.1128/AEM.72.2.1705-1707.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Sunyakumthorn, P., Bourchookarn, A., Pornwiroon, W., David, C., Barker, S. A., Macaluso, K. R. (2008). Characterization and Growth of Polymorphic Rickettsia felis in a Tick Cell Line. Appl. Environ. Microbiol. 74: 3151-3158 [Abstract] [Full Text]  
  • Sakamoto, J. M., Azad, A. F. (2007). Propagation of Arthropod-Borne Rickettsia spp. in Two Mosquito Cell Lines. Appl. Environ. Microbiol. 73: 6637-6643 [Abstract] [Full Text]  
  • Pornwiroon, W., Pourciau, S. S., Foil, L. D., Macaluso, K. R. (2006). Rickettsia felis from Cat Fleas: Isolation and Culture in a Tick-Derived Cell Line. Appl. Environ. Microbiol. 72: 5589-5595 [Abstract] [Full Text]  


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