This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow E-mail this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Stein, C.
Right arrow Articles by Berry, C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Stein, C.
Right arrow Articles by Berry, C.
Agricola
Right arrow Articles by Stein, C.
Right arrow Articles by Berry, C.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, March 2006, p. 1771-1776, Vol. 72, No. 3
0099-2240/06/$08.00+0     doi:10.1128/AEM.72.3.1771-1776.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Transcriptional Analysis of the Toxin-Coding Plasmid pBtoxis from Bacillus thuringiensis subsp. israelensis

Claudia Stein, Gareth W. Jones, Tanya Chalmers, and Colin Berry*

Cardiff School of Biosciences, Cardiff University, Museum Avenue, Cardiff CF10 3US, United Kingdom

Received 13 December 2005/ Accepted 14 December 2005

In Bacillus thuringiensis subsp. israelensis all of the insecticidal toxins are encoded on a single, large plasmid, pBtoxis. Sequencing of this plasmid revealed 125 potential coding sequences, many of which have predicted functions in gene regulation and physiological processes, such as germination. As a first step in understanding the possible role of pBtoxis in its host bacterium, a survey of the transcription of genes with predicted functions was carried out. Whereas many coding sequences, including those previously identified as probable pseudogenes, were not transcribed, mRNA was detected for 29 of the 40 sequences surveyed. Several of these sequences, including eight with similarities to the sequences of known transcriptional regulators, may influence wider gene regulation and thus may alter the phenotype of the host bacterium.

* Corresponding author. Mailing address: Cardiff School of Biosciences, Cardiff University, Museum Avenue, Cardiff CF10 3US, United Kingdom. Phone: 44-29-20874508. Fax: 44-29-20874116. E-mail: Berry{at}cf.ac.uk.

Applied and Environmental Microbiology, March 2006, p. 1771-1776, Vol. 72, No. 3
0099-2240/06/$08.00+0     doi:10.1128/AEM.72.3.1771-1776.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Abdoarrahem, M. M., Gammon, K., Dancer, B. N., Berry, C. (2009). Genetic Basis for Alkaline Activation of Germination in Bacillus thuringiensis subsp. israelensis. Appl. Environ. Microbiol. 75: 6410-6413 [Abstract] [Full Text]  
  • Tang, M., Bideshi, D. K., Park, H.-W., Federici, B. A. (2007). Iteron-Binding ORF157 and FtsZ-Like ORF156 Proteins Encoded by pBtoxis Play a Role in Its Replication in Bacillus thuringiensis subsp. israelensis. J. Bacteriol. 189: 8053-8058 [Abstract] [Full Text]  
  • Manasherob, R., Itsko, M., Sela-Baranes, N., Ben-Dov, E., Berry, C., Cohen, S., Zaritsky, A. (2006). Cyt1Ca from Bacillus thuringiensis subsp. israelensis: production in Escherichia coli and comparison of its biological activities with those of other Cyt-like proteins.. Microbiology 152: 2651-2659 [Abstract] [Full Text]  
  • Gammon, K., Jones, G. W., Hope, S. J., de Oliveira, C. M. F., Regis, L., Silva Filha, M. H. N. L., Dancer, B. N., Berry, C. (2006). Conjugal Transfer of a Toxin-Coding Megaplasmid from Bacillus thuringiensis subsp. israelensis to Mosquitocidal Strains of Bacillus sphaericus.. Appl. Environ. Microbiol. 72: 1766-1770 [Abstract] [Full Text]  


Copyright © 2010 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»