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Applied and Environmental Microbiology, March 2006, p. 1925-1931, Vol. 72, No. 3
0099-2240/06/$08.00+0     doi:10.1128/AEM.72.3.1925-1931.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Supplementation of the Diet with High-Viscosity Beta-Glucan Results in Enrichment for Lactobacilli in the Rat Cecum

Jennifer Snart,¹ Rodrigo Bibiloni,¹ Teresa Grayson,¹ Christophe Lay,² Haiyan Zhang,¹ Gwen E. Allison,^1,3 Julie K. Laverdiere,¹ Feral Temelli,¹ Thavaratnam Vasanthan,¹ Rhonda Bell,¹ and Gerald W. Tannock^1,2*

Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, Alberta, Canada,¹ Department of Microbiology and Immunology, University of Otago, Dunedin, New Zealand,² School of Biochemistry and Molecular Biology, The Australian National University, Canberra, Australia³

Received 27 October 2005/ Accepted 28 December 2005

BBn (BioBreeding) rats were fed casein-based diets supplemented with barley flour, oatmeal flour, cellulose, or barley ß-glucans of high [HV] or low viscosity [LV] in order to measure the prebiotic effects of these different sources of dietary fiber. The dietary impact on the composition of the cecal microbiota was determined by the generation of denaturing gradient gel electrophoresis (DGGE) profiles of PCR-amplified 16S rRNA gene sequences. The DGGE profiles produced from the cecal microbiota of rats within each dietary group were similar, but consensus profiles generated from pooled bacterial DNAs showed differences between rat groups. Animals fed HV glucans (HV-fed rats) had DGGE consensus profiles that were 30% dissimilar from those of the other rat groups. A 16S rRNA gene fragment that was more conspicuous in the profiles of HV-fed animals than in those of cellulose-fed rats had sequence identity with Lactobacillus acidophilus. Measurements of L. acidophilus rRNA abundance (DNA-RNA hybridization), the preparation of cloned 16S rRNA gene libraries, and the enumeration of Lactobacillus cells (fluorescent in situ hybridization) showed that lactobacilli formed a greater proportion of the cecal microbiota in HV-fed rats. In vitro experiments confirmed that some lactobacilli utilize oligosaccharides (degree of polymerization, 3 or 4) present in ß-glucan hydrolysates. The results of this study have relevance to the use of purified ß-glucan products as dietary supplements for human consumption.

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* Corresponding author. Mailing address: Department of Microbiology and Immunology, University of Otago, P.O. Box 56, Dunedin, New Zealand. Phone: 64 3 479 7713. Fax: 64 3 478 8540. E-mail: gerald.tannock{at}stonebow.otago.ac.nz.

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Applied and Environmental Microbiology, March 2006, p. 1925-1931, Vol. 72, No. 3
0099-2240/06/$08.00+0     doi:10.1128/AEM.72.3.1925-1931.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

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