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Applied and Environmental Microbiology, March 2006, p. 2102-2109, Vol. 72, No. 3
0099-2240/06/$08.00+0     doi:10.1128/AEM.72.3.2102-2109.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Denitrifier Community Composition along a Nitrate and Salinity Gradient in a Coastal Aquifer

Alyson E. Santoro,¹ Alexandria B. Boehm,¹ and Christopher A. Francis^2*

Department of Civil and Environmental Engineering, Stanford University, Stanford, California 94305,¹ Department of Geological and Environmental Sciences, Stanford University, Stanford, California 94305²

Received 11 August 2005/ Accepted 28 December 2005

Nitrogen flux into the coastal environment via submarine groundwater discharge may be modulated by microbial processes such as denitrification, but the spatial scales at which microbial communities act and vary are not well understood. In this study, we examined the denitrifying community within the beach aquifer at Huntington Beach, California, where high-nitrate groundwater is a persistent feature. Nitrite reductase-encoding gene fragments (nirK and nirS), responsible for the key step in the denitrification pathway, were PCR amplified, cloned, and sequenced from DNAs extracted from aquifer sediments collected along a cross-shore transect, where groundwater ranged in salinity from 8 to 34 practical salinity units and in nitrate concentration from 0.5 to 330 µM. We found taxonomically rich and novel communities, with all nirK clones exhibiting <85% identity and nirS clones exhibiting <92% identity at the amino acid level to those of cultivated denitrifiers and other environmental clones in the database. Unique communities were found at each site, despite being located within 40 m of each other, suggesting that the spatial scale at which denitrifier diversity and community composition vary is small. Statistical analyses of nir sequences using the Monte Carlo-based program -Libshuff confirmed that some populations were indeed distinct, although further sequencing would be required to fully characterize the highly diverse denitrifying communities at this site.

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* Corresponding author. Mailing address: Department of Geological and Environmental Sciences, Building 320, Room 118, Stanford University, Stanford, CA 94305-2115. Phone: (650) 724-0301. Fax: (650) 725-2199. E-mail: caf{at}stanford.edu.

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Applied and Environmental Microbiology, March 2006, p. 2102-2109, Vol. 72, No. 3
0099-2240/06/$08.00+0     doi:10.1128/AEM.72.3.2102-2109.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

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