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Applied and Environmental Microbiology, March 2006, p. 2170-2177, Vol. 72, No. 3
0099-2240/06/$08.00+0     doi:10.1128/AEM.72.3.2170-2177.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Heterologous Expression of BetL, a Betaine Uptake System, Enhances the Stress Tolerance of Lactobacillus salivarius UCC118

Vivien M. Sheehan, Roy D. Sleator, Gerald F. Fitzgerald,* and Colin Hill

Department of Microbiology and Alimentary Pharmabiotic Centre, University College, Cork, Ireland

Received 11 July 2005/ Accepted 27 December 2005

Given the increasing commercial and clinical relevance of probiotic cultures, improving the technological robustness of what are often process-sensitive cultures is an important biological goal. The nisin-controlled expression system was used to direct the heterologous expression of the listerial betaine uptake system BetL in the probiotic strain Lactobacillus salivarius UCC118. Following nisin induction, strains expressing betL exhibited a significant increase in resistance to several stresses, including elevated osmo-, cryo-, baro-, and chill tolerance, as well as increased resistance to spray- and freeze-drying. The ability to confer additional stress tolerance on a probiotic culture may be an important step in delivering viable cultures for maximal efficacy.

* Corresponding author. Mailing address: Department of Microbiology, University College, Cork, Ireland. Phone: 353-21-4902396. Fax: 353-21-4903101. E-mail: g.fitzgerald{at}ucc.ie.

Applied and Environmental Microbiology, March 2006, p. 2170-2177, Vol. 72, No. 3
0099-2240/06/$08.00+0     doi:10.1128/AEM.72.3.2170-2177.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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