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Applied and Environmental Microbiology, March 2006, p. 2221-2225, Vol. 72, No. 3
0099-2240/06/$08.00+0     doi:10.1128/AEM.72.3.2221-2225.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Evaluation of 23S rRNA PCR Primers for Use in Phylogenetic Studies of Bacterial Diversity{dagger}

Dana E. Hunt, Vanja Klepac-Ceraj,{ddagger} Silvia G. Acinas, Clement Gautier,§ Stefan Bertilsson, and Martin F. Polz*

Department of Civil and Environmental Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139

Received 8 September 2005/ Accepted 19 December 2005

The availability of a diverse set of 23S rRNA gene sequences enabled evaluation of the specificity of 39 previously published and 4 newly designed primers specific for bacteria. An extensive clone library constructed using an optimized primer pair resulted in similar gene richness but slightly differing coverage of some phylogenetic groups, compared to a 16S rRNA gene library from the same environmental sample.

* Corresponding author. Mailing address: Massachusetts Institute of Technology, 48-421, 77 Massachusetts Ave., Cambridge, MA 02139. Phone: (617) 253-7128. Fax: (617) 258-8850. E-mail: mpolz{at}mit.edu.

{dagger} Supplemental material for this article may be found at http://aem.asm.org/.

{ddagger} Present address: Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, MA 02115.

§ Present address: Laboratory for Optics and Biosciences, Institut National de la Santé et de la Recherche Médicale, Ecole Polytechnique, 91128 Palaiseau Cedex, France.

Present address: Limnology/Department of Ecology and Evolution, Norbyvägen, Uppsala University, SE-75236 Uppsala, Sweden.

Applied and Environmental Microbiology, March 2006, p. 2221-2225, Vol. 72, No. 3
0099-2240/06/$08.00+0     doi:10.1128/AEM.72.3.2221-2225.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.





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Copyright © 2006 by the American Society for Microbiology. All rights reserved.