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Applied and Environmental Microbiology, March 2006, p. 2247-2253, Vol. 72, No. 3
0099-2240/06/$08.00+0     doi:10.1128/AEM.72.3.2247-2253.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Resistance Determinants of a Highly Arsenic-Resistant Strain of Leptospirillum ferriphilum Isolated from a Commercial Biooxidation Tank

I. Marla Tuffin, Stanton B. Hector, Shelly M. Deane, and Douglas E. Rawlings*

Department of Microbiology, Stellenbosch University, Private Bag X1, Matieland 7602, South Africa

Received 21 October 2005/ Accepted 12 December 2005

Two sets of arsenic resistance genes were isolated from the highly arsenic-resistant Leptospirillum ferriphilum Fairview strain. One set is located on a transposon, TnLfArs, and is related to the previously identified TnAtcArs from Acidithiobacillus caldus isolated from the same arsenopyrite biooxidation tank as L. ferriphilum. TnLfArs conferred resistance to arsenite and arsenate and was transpositionally active in Escherichia coli. TnLfArs and TnAtcArs were sufficiently different for them not to have been transferred from one type of bacterium to the other in the biooxidation tank. The second set of arsenic resistance genes conferred very low levels of resistance in E. coli and appeared to be poorly expressed in both L. ferriphilum and E. coli.

* Corresponding author. Mailing address: Department of Microbiology, Stellenbosch University, Private Bag X1, Matieland 7602, South Africa. Phone: 27-21-808 5848. Fax: 27-21-808 5846. E-mail: der{at}sun.ac.za.

Applied and Environmental Microbiology, March 2006, p. 2247-2253, Vol. 72, No. 3
0099-2240/06/$08.00+0     doi:10.1128/AEM.72.3.2247-2253.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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