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Applied and Environmental Microbiology, March 2006, p. 2283-2286, Vol. 72, No. 3
0099-2240/06/$08.00+0     doi:10.1128/AEM.72.3.2283-2286.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Conversion of Sphingobium chlorophenolicum ATCC 39723 to a Hexachlorobenzene Degrader by Metabolic Engineering

Da-Zhong Yan,1,2 Hong Liu,1 and Ning-Yi Zhou1*

Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China,1 Graduate School, Chinese Academy of Sciences, Beijing 100039, China2

Received 11 October 2005/ Accepted 5 December 2005

The gene cassette (camA+ camB+ camC) encoding a cytochrome P-450cam variant was integrated into the nonessential gene pcpM of the pentachlorophenol degrader Sphingobium chlorophenolicum ATCC 39723 by homologous recombination. The recombinant strain could degrade hexachlorobenzene at a rate of 0.67 nmol · mg (dry weight)–1 · h–1, and intermediate pentachlorophenol was also identified.

* Corresponding author. Mailing address: Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China. Phone: 86-27-87197655. Fax: 86-27-87197655. E-mail: n.zhou{at}pentium.whiov.ac.cn.

Applied and Environmental Microbiology, March 2006, p. 2283-2286, Vol. 72, No. 3
0099-2240/06/$08.00+0     doi:10.1128/AEM.72.3.2283-2286.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Takagi, K., Iwasaki, A., Kamei, I., Satsuma, K., Yoshioka, Y., Harada, N. (2009). Aerobic Mineralization of Hexachlorobenzene by Newly Isolated Pentachloronitrobenzene-Degrading Nocardioides sp. Strain PD653. Appl. Environ. Microbiol. 75: 4452-4458 [Abstract] [Full Text]  
  • Xu, F., Bell, S. G., Rao, Z., Wong, L.-L. (2007). Structure-activity correlations in pentachlorobenzene oxidation by engineered cytochrome P450cam. Protein Eng Des Sel 0: gzm028v1-8 [Abstract] [Full Text]  


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