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Applied and Environmental Microbiology, April 2006, p. 2373-2378, Vol. 72, No. 4
0099-2240/06/$08.00+0     doi:10.1128/AEM.72.4.2373-2378.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Genotyping and Pathogenicity of Viral Hemorrhagic Septicemia Virus from Free-Living Turbot (Psetta maxima) in a Turkish Coastal Area of the Black Sea

Toyohiko Nishizawa,1* Haci Savas,2 Hakan Isidan,2,3 Cennet Üstündag,2 Hiroshi Iwamoto,4 and Mamoru Yoshimizu1

Graduate School of Fisheries Sciences, Hokkaido University, Hakodate 041-8611, Japan,1 Central Fisheries Research Institute, Ministry of Agriculture and Rural Affairs, Trabzon 61250, Turkey,2 Faculty of Veterinary Medicine, University of Firat, Elazig 23119, Turkey,3 Japan International Cooperation Agency, Yoyogi 2-1-1, Shibuya-ku, Tokyo 151-8558, Japan4

Received 12 August 2005/ Accepted 17 January 2006

Viral hemorrhagic septicemia (VHS) is one of the most serious fish viral diseases for cultured rainbow trout (Oncorhynchus mykiss), although VHS virus (VHSV) seems to be ubiquitous among marine fishes. In the present study, VHSV isolation was performed with free-living and cultured turbot (Psetta maxima) in the Trabzon coastal area of the Black Sea to evaluate participation of VHSV in mass mortalities of seed-produced turbot larvae. VHSV was detected in 14 of 66 free-living spawners (positive ratio, 21.2%), 1 of 65 free-living immature fish (1.5%) and 7 of 40 cultured brood stock (17.5%), respectively. Based on a partial glycoprotein gene nucleotide sequence, Turkish VHSV isolates were classified into the class I-e of genotype I and were the most closely related to the GE-1.2 isolate (>98% identity), which was found >20 years ago in Georgia. Thus, it was revealed that Turkish VHSV isolates were not introduced from European countries, it could be an indigenous type of VHSV distributing in the Black Sea environment. In pathogenicity tests, the Turkish isolates did not induce mortality in turbot larvae and rainbow trout fingerlings. Mass mortalities at a rate of approximately 90% occurred in turbot larvae produced by experimental seeding, although VHSV was not detected in any dead fish. Thus, it was concluded that mass mortality in the seed-produced turbot larvae was not caused by VHSV infection.

* Corresponding author. Mailing address: Faculty of Fisheries Sciences, Hokkaido University, Hakodate 041-8611, Japan. Phone and fax: 81-138-40-8881. E-mail: jjnishi{at}fish.hokudai.ac.jp.

Applied and Environmental Microbiology, April 2006, p. 2373-2378, Vol. 72, No. 4
0099-2240/06/$08.00+0     doi:10.1128/AEM.72.4.2373-2378.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.





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