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Applied and Environmental Microbiology, April 2006, p. 2476-2482, Vol. 72, No. 4
0099-2240/06/$08.00+0     doi:10.1128/AEM.72.4.2476-2482.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Degradation of Aroclor 1242 Dechlorination Products in Sediments by Burkholderia xenovorans LB400(ohb) and Rhodococcus sp. Strain RHA1(fcb)

Jorge L. M. Rodrigues,1,2,3 C. Alan Kachel,1 Michael R. Aiello,1,4 John F. Quensen,1,2 Olga V. Maltseva,1,2 Tamara V. Tsoi,1,2 and James M. Tiedje1,2,3,4*

NSF Center for Microbial Ecology,1 Department of Crop and Soil Sciences,2 Institute for Environmental Toxicology,3 Department of Microbiology and Molecular Genetics, Michigan State University, East Lansing, Michigan 488244

Received 7 October 2005/ Accepted 20 January 2006

Burkholderia xenovorans strain LB400, which possesses the biphenyl pathway, was engineered to contain the oxygenolytic ortho dehalogenation (ohb) operon, allowing it to grow on 2-chlorobenzoate and to completely mineralize 2-chlorobiphenyl. A two-stage anaerobic/aerobic biotreatment process for Aroclor 1242-contaminated sediment was simulated, and the degradation activities and genetic stabilities of LB400(ohb) and the previously constructed strain RHA1(fcb), capable of growth on 4-chlorobenzoate, were monitored during the aerobic phase. The population dynamics of both strains were also followed by selective plating and real-time PCR, with comparable results; populations of both recombinants increased in the contaminated sediment. Inoculation at different cell densities (104 or 106 cells g–1 sediment) did not affect the extent of polychlorinated biphenyl (PCB) biodegradation. After 30 days, PCB removal rates for high and low inoculation densities were 57% and 54%, respectively, during the aerobic phase.

* Corresponding author. Mailing address: Center for Microbial Ecology, 540 Plant and Soil Science Building, Michigan State University, East Lansing, MI 48824. Phone: (517) 353-7858. Fax: (517) 353-2917. E-mail: tiedjej{at}msu.edu.

Applied and Environmental Microbiology, April 2006, p. 2476-2482, Vol. 72, No. 4
0099-2240/06/$08.00+0     doi:10.1128/AEM.72.4.2476-2482.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Parnell, J. J., Park, J., Denef, V., Tsoi, T., Hashsham, S., Quensen, J. III, Tiedje, J. M. (2006). Coping with Polychlorinated Biphenyl (PCB) Toxicity: Physiological and Genome-Wide Responses of Burkholderia xenovorans LB400 to PCB-Mediated Stress. Appl. Environ. Microbiol. 72: 6607-6614 [Abstract] [Full Text]  


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