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Applied and Environmental Microbiology, April 2006, p. 2672-2678, Vol. 72, No. 4
0099-2240/06/$08.00+0     doi:10.1128/AEM.72.4.2672-2678.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Both msa Genes in Renibacterium salmoninarum Are Needed for Full Virulence in Bacterial Kidney Disease

Alison M. Coady,1 Anthony L. Murray,2 Diane G. Elliott,2 and Linda D. Rhodes1*

Northwest Fisheries Science Center, 2725 Montlake Boulevard East, Seattle, Washington 98112,1 Western Fisheries Research Center, U.S. Geological Survey, 6505 Northeast 65th Street, Seattle, Washington 981152

Received 10 September 2005/ Accepted 5 February 2006

Renibacterium salmoninarum, a gram-positive diplococcobacillus that causes bacterial kidney disease among salmon and trout, has two chromosomal loci encoding the major soluble antigen (msa) gene. Because the MSA protein is widely suspected to be an important virulence factor, we used insertion-duplication mutagenesis to generate disruptions of either the msa1 or msa2 gene. Surprisingly, expression of MSA protein in broth cultures appeared unaffected. However, the virulence of either mutant in juvenile chinook salmon (Oncorhynchus tshawytscha) by intraperitoneal challenge was severely attenuated, suggesting that disruption of the msa1 or msa2 gene affected in vivo expression.

* Corresponding author. Mailing address: Northwest Fisheries Science Center, 2725 Montlake Blvd. East, Seattle, WA 98112. Phone: (206) 860-3279. Fax: (206) 860-3467. E-mail: linda.rhodes{at}noaa.gov.

Applied and Environmental Microbiology, April 2006, p. 2672-2678, Vol. 72, No. 4
0099-2240/06/$08.00+0     doi:10.1128/AEM.72.4.2672-2678.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Wiens, G. D., Rockey, D. D., Wu, Z., Chang, J., Levy, R., Crane, S., Chen, D. S., Capri, G. R., Burnett, J. R., Sudheesh, P. S., Schipma, M. J., Burd, H., Bhattacharyya, A., Rhodes, L. D., Kaul, R., Strom, M. S. (2008). Genome Sequence of the Fish Pathogen Renibacterium salmoninarum Suggests Reductive Evolution away from an Environmental Arthrobacter Ancestor. J. Bacteriol. 190: 6970-6982 [Abstract] [Full Text]  


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