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Applied and Environmental Microbiology, April 2006, p. 3011-3015, Vol. 72, No. 4
0099-2240/06/$08.00+0     doi:10.1128/AEM.72.4.3011-3015.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Culture Conditions of Roseobacter Strain 27-4 Affect Its Attachment and Biofilm Formation as Quantified by Real-Time PCR

Jesper Bartholin Bruhn,1* Janus Anders Juul Haagensen,2 Dorthe Bagge-Ravn,1,{dagger} and Lone Gram1

Danish Institute for Fisheries Research, Department of Seafood Research, Søltofts Plads, DK-2800 Kongens Lyngby, Denmark,1 Center for Biomedical Microbiology, BioCentrum, Technical University of Denmark, DK-2800 Kongens Lyngby, Denmark2

Received 14 July 2005/ Accepted 31 December 2005

The fish probiotic bacterium Roseobacter strain 27-4 grows only as rosettes and produces its antibacterial compound under static growth conditions. It forms three-dimensional biofilms when precultured under static conditions. We quantified attachment of Roseobacter strain 27-4 using a direct real-time PCR method and demonstrated that the bacteria attached more efficiently to surfaces during static growth than under aerated conditions.

* Corresponding author. Mailing address: Department of Seafood Research, Danish Institute for Fisheries Research, Søltofts Plads, DTU Bldg. 221, DK-2800 Kgs. Lyngby, Denmark. Phone: 45 45 25 25 71. Fax: 45 45 88 47 74. E-mail: jbb{at}dfu.min.dk.

{dagger} Present address: Chr Hansen A/S, Sdr. Ringvej 22, DK-4000 Roskilde, Denmark.

Applied and Environmental Microbiology, April 2006, p. 3011-3015, Vol. 72, No. 4
0099-2240/06/$08.00+0     doi:10.1128/AEM.72.4.3011-3015.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Slightom, R. N., Buchan, A. (2009). Surface Colonization by Marine Roseobacters: Integrating Genotype and Phenotype. Appl. Environ. Microbiol. 75: 6027-6037 [Abstract] [Full Text]  
  • Waidner, L. A., Kirchman, D. L. (2007). Aerobic Anoxygenic Phototrophic Bacteria Attached to Particles in Turbid Waters of the Delaware and Chesapeake Estuaries. Appl. Environ. Microbiol. 73: 3936-3944 [Abstract] [Full Text]  
  • Bruhn, J. B., Gram, L., Belas, R. (2007). Production of Antibacterial Compounds and Biofilm Formation by Roseobacter Species Are Influenced by Culture Conditions. Appl. Environ. Microbiol. 73: 442-450 [Abstract] [Full Text]  


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