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Applied and Environmental Microbiology, April 2006, p. 3016-3020, Vol. 72, No. 4
0099-2240/06/$08.00+0     doi:10.1128/AEM.72.4.3016-3020.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Fluorescence In Situ Hybridization and Spectral Imaging of Coral-Associated Bacterial Communities

T. D. Ainsworth,1* M. Fine,1,2 L. L. Blackall,3 and O. Hoegh-Guldberg1

Centre for Marine Studies, The University of Queensland, St. Lucia, QLD 4072, Australia,1 The Leon Recanati Institute for Maritime Studies, University of Haifa, Mount Carmel, Haifa 31905, Israel,2 Advanced Wastewater Management Centre, The University of Queensland, St. Lucia, QLD 4072, Australia3

Received 6 December 2005/ Accepted 17 January 2006

Microbial communities play important roles in the functioning of coral reef communities. However, extensive autofluorescence of coral tissues and endosymbionts limits the application of standard fluorescence in situ hybridization (FISH) techniques for the identification of the coral-associated bacterial communities. This study overcomes these limitations by combining FISH and spectral imaging.

* Corresponding author. Mailing address: Centre for Marine Studies, The University of Queensland, St. Lucia, Brisbane, QLD 4072, Australia. Phone: 617 3379 6858 Fax: 61 7 3365 4755. E-mail: t.ainsworth{at}uq.edu.au.

Applied and Environmental Microbiology, April 2006, p. 3016-3020, Vol. 72, No. 4
0099-2240/06/$08.00+0     doi:10.1128/AEM.72.4.3016-3020.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Polerecky, L., Bissett, A., Al-Najjar, M., Faerber, P., Osmers, H., Suci, P. A., Stoodley, P., de Beer, D. (2009). Modular Spectral Imaging System for Discrimination of Pigments in Cells and Microbial Communities. Appl. Environ. Microbiol. 75: 758-771 [Abstract] [Full Text]  
  • Ainsworth, T. D., Kramasky-Winter, E., Loya, Y., Hoegh-Guldberg, O., Fine, M. (2007). Coral Disease Diagnostics: What's between a Plague and a Band?. Appl. Environ. Microbiol. 73: 981-992 [Abstract] [Full Text]  


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