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Applied and Environmental Microbiology, April 2006, p. 3062-3065, Vol. 72, No. 4
0099-2240/06/$08.00+0     doi:10.1128/AEM.72.4.3062-3065.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Produce Isolates of the Escherichia coli Ont:H52 Serotype That Carry both Shiga Toxin 1 and Stable Toxin Genes

Steven R. Monday,1 Christina Keys,1 Patricia Hanson,2 Yuelian Shen,2 Thomas S. Whittam,3 and Peter Feng1*

Division of Microbiological Studies, U.S. Food and Drug Administration, College Park, Maryland 20740,1 Florida Department of Agriculture and Consumer Services, Tallahassee, Florida 32339,2 STEC Center, Michigan State University, East Lansing, Michigan 488243

Received 24 October 2005/ Accepted 2 February 2006

Produce isolates of the Escherichia coli Ont:H52 serotype carried Shiga toxin 1 and stable toxin genes but only expressed Stx1. These strains had pulsed-field gel electrophoresis profiles that were 90% homologous to clinical Ont:H52 strains that had identical phenotypes and genotypes. All Ont:H52 strains had identical single nucleotide polymorphism profiles that are suggestive of a unique clonal group.

* Corresponding author. Mailing address: HFS-516, FDA, 5100 Paint Branch Parkway, College Park, MD 20740. Phone: (301) 436-1650. Fax: (301) 436-2644. E-mail: pfeng{at}cfsan.fda.gov.

Applied and Environmental Microbiology, April 2006, p. 3062-3065, Vol. 72, No. 4
0099-2240/06/$08.00+0     doi:10.1128/AEM.72.4.3062-3065.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.





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