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Applied and Environmental Microbiology, April 2006, p. 3066-3068, Vol. 72, No. 4
0099-2240/06/$08.00+0     doi:10.1128/AEM.72.4.3066-3068.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

PslD Is a Secreted Protein Required for Biofilm Formation by Pseudomonas aeruginosa

Andrea Campisano, Christine Schroeder, Mirle Schemionek, Joerg Overhage, and Bernd H. A. Rehm*

Institute of Molecular Biosciences, Massey University, Private Bag 11222, Palmerston North, New Zealand

Received 15 December 2005/ Accepted 3 February 2006

The function of pslD, which is part of the psl operon from Pseudomonas aeruginosa, was investigated in this study. The psl operon is involved in exopolysaccharide biosynthesis and biofilm formation. An isogenic marker-free pslD deletion mutant of P. aeruginosa PAO1 which was deficient in the formation of differentiated biofilms was generated. Expression of only the pslD gene coding region restored the wild-type phenotype. A C-terminal, hexahistidine tag fusion enabled the identification of PslD. LacZ and PhoA translational fusions with PslD indicated that PslD is a secreted protein required for biofilm formation, presumably via its role in exopolysaccharide export.

* Corresponding author. Mailing address: Institute of Molecular Sciences, Massey University, Private Bag 11222, Palmerston North, New Zealand. Phone: 64 6 350 5515 7890. Fax: 64 6 350 5688. E-mail: B.Rehm{at}massey.ac.nz.

Applied and Environmental Microbiology, April 2006, p. 3066-3068, Vol. 72, No. 4
0099-2240/06/$08.00+0     doi:10.1128/AEM.72.4.3066-3068.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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