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Applied and Environmental Microbiology, May 2006, p. 3259-3267, Vol. 72, No. 5
0099-2240/06/$08.00+0     doi:10.1128/AEM.72.5.3259-3267.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Glycosylphosphatidylinositol-Anchored Ecm33p Influences Conidial Cell Wall Biosynthesis in Aspergillus fumigatus

Sandrine Chabane,¹ Jacqueline Sarfati,¹ Oumaima Ibrahim-Granet,¹ Chen Du,³ Christine Schmidt,² Isabelle Mouyna,¹ Marie-Christine Prevost,² Richard Calderone,³ and Jean-Paul Latgé^1*

Aspergillus Unit,¹ Electron Microscopy Platform, Institut Pasteur, 25-28 Rue du Dr Roux, 75724 Paris Cedex 15,² Department of Microbiology and Immunology, Georgetown University Medical Center, Washington, D.C. 20007³

Received 22 August 2005/ Accepted 24 February 2006

ECM33 encodes a glycosylphosphatidylinositol-anchored protein whose orthologs in yeast are essential for sporulation. Aspergillus fumigatus Ecm33p is unique and has an apparent mass of 55 kDa. Disruption of A. fumigatus ECM33 results in a mutant with several morphogenetic aberrations, including the following: (i) a defect in conidial separation, (ii) an increase in the diameter of the conidia of the mutant associated with an increase in the concentration of the cell wall chitin, (iii) conidia that were sensitive to the absence of aeration during long-term storage, and (iv) conidia that were more resistant to killing by phagocytes, whereas the mycelium was more easily killed by neutrophils.

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* Corresponding author. Mailing address: Aspergillus Unit, Institut Pasteur, 25 rue du Docteur Roux, 75724 Paris Cedex 15, France. Phone: 145688225. Fax: 140613419. E-mail: jplatge{at}pasteur.fr.

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Applied and Environmental Microbiology, May 2006, p. 3259-3267, Vol. 72, No. 5
0099-2240/06/$08.00+0     doi:10.1128/AEM.72.5.3259-3267.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

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