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Applied and Environmental Microbiology, May 2006, p. 3309-3313, Vol. 72, No. 5
0099-2240/06/$08.00+0     doi:10.1128/AEM.72.5.3309-3313.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Genetic Diversity and Quinolone Resistance in Campylobacter jejuni Isolates from Poultry in Senegal

Alfred Dieudonné Kinana,1* Eric Cardinale,2 Fatou Tall,3 Ibrahim Bahsoun,1 Jean-Marie Sire,1 Benoit Garin,1 Sebastien Breurec,1 Cheikh Saad-Bouh Boye,4 and Jean-David Perrier-Gros-Claude5

Laboratoire de biologie médicale, Institut Pasteur de Dakar, Dakar, Sénégal,1 Programme Productions animales, CIRAD-EMVT, TA/30A Campus international de Baillarguet, 34398 Montpellier Cedex 5, France,2 ISRA-LNERV, BP 2057, Dakar-Hann, Sénégal,3 Hôpital Aristide Le Dantec, Dakar, Sénégal,4 Institut Pasteur du Maroc, Casablanca, Maroc5

Received 18 October 2005/ Accepted 4 February 2006

We used the multilocus sequence typing (MLST) method to evaluate the genetic diversity of 46 Campylobacter jejuni isolates from chickens and to determine the link between quinolone resistance and sequence type (ST). There were a total of 16 ST genotypes, and the majority of them belonged to seven clonal complexes previously identified by using isolates from human disease. The ST-353 complex was the most common complex, whereas the ST-21, ST-42, ST-52, and ST-257 complexes were less well represented. The resistance phenotype varied for each ST, and the Thr-86-Ile substitution in the GyrA protein was the predominant mechanism of resistance to quinolone. Nine of the 14 isolates having the Thr-86-Ile substitution belonged to the ST-353 complex. MLST showed that the emergence of quinolone resistance is not related to the diffusion of a unique clone and that there is no link between ST genotype and quinolone resistance. Based on silent mutations, different variants of the gyrA gene were shown to exist for the same ST. These data provide useful information for understanding the epidemiology of C. jejuni in Senegal.

* Corresponding author. Mailing address: Laboratoire de biologie médicale, Institut Pasteur de Dakar, 36 avenue Pasteur, Dakar, Sénégal. Phone: (221) 839 92 00. Fax: (221) 822 70 52. E-mail: kinana{at}pasteur.sn.

Applied and Environmental Microbiology, May 2006, p. 3309-3313, Vol. 72, No. 5
0099-2240/06/$08.00+0     doi:10.1128/AEM.72.5.3309-3313.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.





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Copyright © 2006 by the American Society for Microbiology. All rights reserved.