This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kinana, A. D.
Right arrow Articles by Perrier-Gros-Claude, J.-D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kinana, A. D.
Right arrow Articles by Perrier-Gros-Claude, J.-D.
Agricola
Right arrow Articles by Kinana, A. D.
Right arrow Articles by Perrier-Gros-Claude, J.-D.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, May 2006, p. 3309-3313, Vol. 72, No. 5
0099-2240/06/$08.00+0     doi:10.1128/AEM.72.5.3309-3313.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Genetic Diversity and Quinolone Resistance in Campylobacter jejuni Isolates from Poultry in Senegal

Alfred Dieudonné Kinana,1* Eric Cardinale,2 Fatou Tall,3 Ibrahim Bahsoun,1 Jean-Marie Sire,1 Benoit Garin,1 Sebastien Breurec,1 Cheikh Saad-Bouh Boye,4 and Jean-David Perrier-Gros-Claude5

Laboratoire de biologie médicale, Institut Pasteur de Dakar, Dakar, Sénégal,1 Programme Productions animales, CIRAD-EMVT, TA/30A Campus international de Baillarguet, 34398 Montpellier Cedex 5, France,2 ISRA-LNERV, BP 2057, Dakar-Hann, Sénégal,3 Hôpital Aristide Le Dantec, Dakar, Sénégal,4 Institut Pasteur du Maroc, Casablanca, Maroc5

Received 18 October 2005/ Accepted 4 February 2006

We used the multilocus sequence typing (MLST) method to evaluate the genetic diversity of 46 Campylobacter jejuni isolates from chickens and to determine the link between quinolone resistance and sequence type (ST). There were a total of 16 ST genotypes, and the majority of them belonged to seven clonal complexes previously identified by using isolates from human disease. The ST-353 complex was the most common complex, whereas the ST-21, ST-42, ST-52, and ST-257 complexes were less well represented. The resistance phenotype varied for each ST, and the Thr-86-Ile substitution in the GyrA protein was the predominant mechanism of resistance to quinolone. Nine of the 14 isolates having the Thr-86-Ile substitution belonged to the ST-353 complex. MLST showed that the emergence of quinolone resistance is not related to the diffusion of a unique clone and that there is no link between ST genotype and quinolone resistance. Based on silent mutations, different variants of the gyrA gene were shown to exist for the same ST. These data provide useful information for understanding the epidemiology of C. jejuni in Senegal.

* Corresponding author. Mailing address: Laboratoire de biologie médicale, Institut Pasteur de Dakar, 36 avenue Pasteur, Dakar, Sénégal. Phone: (221) 839 92 00. Fax: (221) 822 70 52. E-mail: kinana{at}pasteur.sn.

Applied and Environmental Microbiology, May 2006, p. 3309-3313, Vol. 72, No. 5
0099-2240/06/$08.00+0     doi:10.1128/AEM.72.5.3309-3313.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Kinana, A. D., Ricci, V., Piddock, L. J. V. (2009). Contribution of efflux to antibiotic resistance in Campylobacter isolated from poultry in Senegal. J Antimicrob Chemother 0: dkp235v1-dkp235 [Full Text]  
  • Levesque, S., Frost, E., Arbeit, R. D., Michaud, S. (2008). Multilocus Sequence Typing of Campylobacter jejuni Isolates from Humans, Chickens, Raw Milk, and Environmental Water in Quebec, Canada. J. Clin. Microbiol. 46: 3404-3411 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»