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Applied and Environmental Microbiology, May 2006, p. 3330-3335, Vol. 72, No. 5
0099-2240/06/$08.00+0     doi:10.1128/AEM.72.5.3330-3335.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

L-Methionine Degradation Pathway in Kluyveromyces lactis: Identification and Functional Analysis of the Genes Encoding L-Methionine Aminotransferase

Dafni-Maria Kagkli,1,2,3 Pascal Bonnarme,2* Cécile Neuvéglise,3 Timothy M. Cogan,1 and Serge Casaregola3

Dairy Products Research Centre, Teagasc, Fermoy, Ireland,1 Institut National de la Recherche Agronomique, Unité Mixte de Recherches Génie et Microbiologie des Procédés Alimentaires, CBAI, 78850 Thiverval-Grignon, France,2 Laboratoire de Microbiologie et Génétique Moléculaire et Cellulaire, CNRS-INRA, Institut National Agronomique Paris-Grignon, F-78850 Thiverval-Grignon, France3

Received 14 December 2005/ Accepted 1 March 2006

Kluyveromyces lactis is one of the cheese-ripening yeasts and is believed to contribute to the formation of volatile sulfur compounds (VSCs) through degradation of L-methionine. L-Methionine aminotransferase is potentially involved in the pathway that results in the production of methanethiol, a common precursor of VSCs. Even though this pathway has been studied previously, the genes involved have never been studied. In this study, on the basis of sequence homology, all the putative aminotransferase-encoding genes from K. lactis were cloned in an overproducing vector, pCXJ10, and their effects on the production of VSCs were analyzed. Two genes, KlARO8.1 and KlARO8.2, were found to be responsible for L-methionine aminotransferase activity. Transformants carrying these genes cloned in the pCXJ10 vector produced threefold-larger amounts of VSCs than the transformant containing the plasmid without any insert or other related putative aminotransferases produced.

* Corresponding author. Mailing address: INRA, Unité Mixte de Recherches Génie et Microbiologie des Procédés Alimentaires, CBAI, 78850 Thiverval-Grignon, France. Phone: 33 1 30 81 53 88. Fax: 33 1 30 81 55 97. E-mail: bonnarme{at}grignon.inra.fr.

Applied and Environmental Microbiology, May 2006, p. 3330-3335, Vol. 72, No. 5
0099-2240/06/$08.00+0     doi:10.1128/AEM.72.5.3330-3335.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Cholet, O., Henaut, A., Hebert, A., Bonnarme, P. (2008). Transcriptional Analysis of L-Methionine Catabolism in the Cheese-Ripening Yeast Yarrowia lipolytica in Relation to Volatile Sulfur Compound Biosynthesis. Appl. Environ. Microbiol. 74: 3356-3367 [Abstract] [Full Text]  
  • Cholet, O., Henaut, A., Casaregola, S., Bonnarme, P. (2007). Gene Expression and Biochemical Analysis of Cheese-Ripening Yeasts: Focus on Catabolism of L-Methionine, Lactate, and Lactose. Appl. Environ. Microbiol. 73: 2561-2570 [Abstract] [Full Text]  


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