This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow E-mail this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Sung, B. H.
Right arrow Articles by Kim, S. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sung, B. H.
Right arrow Articles by Kim, S. C.
Agricola
Right arrow Articles by Sung, B. H.
Right arrow Articles by Kim, S. C.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, May 2006, p. 3336-3342, Vol. 72, No. 5
0099-2240/06/$08.00+0     doi:10.1128/AEM.72.5.3336-3342.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Development of a Biofilm Production-Deficient Escherichia coli Strain as a Host for Biotechnological Applications

Bong Hyun Sung,1,{dagger} Choong Hoon Lee,1,{dagger} Byung Jo Yu,1 Jun Hyoung Lee,1 Ju Young Lee,1 Mi Sun Kim,2 Frederick R. Blattner,3 and Sun Chang Kim1*

Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon 305-701, Korea,1 Biomass Team, Korea Institute of Energy Research, Daejeon 305-343, Korea,2 Department of Genetics, University of Wisconsin, Madison, Wisconsin 537063

Received 15 March 2005/ Accepted 25 February 2006

Bacteria form biofilms by adhering to biotic or abiotic surfaces. This phenomenon causes several problems, including a reduction in the transport of mass and heat, an increase in resistance to antibiotics, and a shortening of the lifetimes of modules in bioindustrial fermentors. To overcome these difficulties, we created a biofilm production-deficient Escherichia coli strain, BD123, by deleting genes involved in curli biosynthesis and assembly, {Delta}(csgG-csgC); colanic acid biosynthesis and assembly, {Delta}(wcaL-wza); and type I pilus biosynthesis, {Delta}(fimB-fimH). E. coli BD123 remained mostly in the form of planktonic cells under the conditions tested and became more sensitive to the antibiotics streptomycin and rifampin than the wild-type E. coli MG1655: the growth of BD123 was inhibited by one-fourth of the concentrations needed to inhibit MG1655. In addition, the transformation efficiency of BD123 was about 20 times higher than that of MG1655, and the production and secretion of recombinant proteins were ~16% and ~25% greater, respectively, with BD123 than with MG1655. These results indicate that the newly created biofilm production-deficient strain of E. coli displays several key properties that substantially enhance its utility in the biotechnology arena.

* Corresponding author. Mailing address: Department of Biological Sciences, Korea Advanced Institute of Science and Technology, 373-1 Guseong-dong Yuseong-gu, Daejeon 305-701, Korea. Phone: 82-42-869-2619. Fax: 82-42-869-2610. E-mail: sunkim{at}kaist.ac.kr.

{dagger} B. H. Sung and C. H. Lee contributed equally to this work.

Applied and Environmental Microbiology, May 2006, p. 3336-3342, Vol. 72, No. 5
0099-2240/06/$08.00+0     doi:10.1128/AEM.72.5.3336-3342.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Lee, J. Y., Sung, B. H., Yu, B. J., Lee, J. H., Lee, S. H., Kim, M. S., Koob, M. D., Kim, S. C. (2008). Phenotypic engineering by reprogramming gene transcription using novel artificial transcription factors in Escherichia coli. Nucleic Acids Res 36: e102-e102 [Abstract] [Full Text]  
  • Yu, B. J., Kang, K. H., Lee, J. H., Sung, B. H., Kim, M. S., Kim, S. C. (2008). Rapid and efficient construction of markerless deletions in the Escherichia coli genome. Nucleic Acids Res 36: e84-e84 [Abstract] [Full Text]  
  • Da Re, S., Le Quere, B., Ghigo, J.-M., Beloin, C. (2007). Tight Modulation of Escherichia coli Bacterial Biofilm Formation through Controlled Expression of Adhesion Factors. Appl. Environ. Microbiol. 73: 3391-3403 [Abstract] [Full Text]  


Copyright © 2010 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»