This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Oda, K.
Right arrow Articles by Iwashita, K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Oda, K.
Right arrow Articles by Iwashita, K.
Agricola
Right arrow Articles by Oda, K.
Right arrow Articles by Iwashita, K.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, May 2006, p. 3448-3457, Vol. 72, No. 5
0099-2240/06/$08.00+0     doi:10.1128/AEM.72.5.3448-3457.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Proteomic Analysis of Extracellular Proteins from Aspergillus oryzae Grown under Submerged and Solid-State Culture Conditions

Ken Oda, Dararat Kakizono, Osamu Yamada, Haruyuki Iefuji, Osamu Akita, and Kazuhiro Iwashita*

National Research Institute of Brewing, 3-7-1 Kagamiyama, Higashi-hiroshima, Hiroshima 739-0046, Japan

Received 31 October 2005/ Accepted 24 February 2006

Filamentous fungi are widely used for the production of homologous and heterologous proteins. Recently, there has been increasing interest in Aspergillus oryzae because of its ability to produce heterologous proteins in solid-state culture. To provide an overview of protein secretion by A. oryzae in solid-state culture, we carried out a comparative proteome analysis of extracellular proteins in solid-state and submerged (liquid) cultures. Extracellular proteins prepared from both cultures sequentially from 0 to 40 h were subjected to two-dimensional electrophoresis, and protein spots at 40 h were identified by peptide mass fingerprinting using matrix-assisted laser desorption ionization-time-of-flight mass spectrometry. We also attempted to identify cell wall-bound proteins of the submerged culture. We analyzed 85 spots from the solid-state culture and 110 spots from the submerged culture. We identified a total of 29 proteins, which were classified into 4 groups. Group 1 consisted of extracellular proteins specifically produced in the solid-state growth condition, such as glucoamylase B and alanyl dipeptidyl peptidase. Group 2 consisted of extracellular proteins specifically produced in the submerged condition, such as glucoamylase A (GlaA) and xylanase G2 (XynG2). Group 3 consisted of proteins produced in both conditions, such as xylanase G1. Group 4 consisted of proteins that were secreted to the medium in the solid-state growth condition but trapped in the cell wall in the submerged condition, such as {alpha}-amylase (TAA) and ß-glucosidase (Bgl). A Northern analysis of seven genes from the four groups suggested that the secretion of TAA and Bgl was regulated by trapping these proteins in the cell wall in submerged culture and that secretion of GlaA and XynG2 was regulated at the posttranscriptional level in the solid-state culture.

* Corresponding author. Mailing address: National Research Institute of Brewing, 3-7-1 Kagamiyama, Higashi-hiroshima, Hiroshima 739-0046, Japan. Phone: 81(824)20-0800. Fax: 81(824)20-0802. E-mail: iwashitact{at}nrib.go.jp.

Applied and Environmental Microbiology, May 2006, p. 3448-3457, Vol. 72, No. 5
0099-2240/06/$08.00+0     doi:10.1128/AEM.72.5.3448-3457.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Zhang, L., Feng, D., Fang, W., Ouyang, H., Luo, Y., Du, T., Jin, C. (2009). Comparative proteomic analysis of an Aspergillus fumigatus mutant deficient in glucosidase I (AfCwh41). Microbiology 155: 2157-2167 [Abstract] [Full Text]  
  • Machida, M., Yamada, O., Gomi, K. (2008). Genomics of Aspergillus oryzae: Learning from the History of Koji Mold and Exploration of Its Future. DNA Res 15: 173-183 [Abstract] [Full Text]  
  • Kim, Y., Nandakumar, M. P., Marten, M. R. (2008). The state of proteome profiling in the fungal genus Aspergillus. Brief Funct Genomic Proteomic 7: 87-94 [Abstract] [Full Text]  
  • Rodrigues, M. L., Nakayasu, E. S., Oliveira, D. L., Nimrichter, L., Nosanchuk, J. D., Almeida, I. C., Casadevall, A. (2008). Extracellular Vesicles Produced by Cryptococcus neoformans Contain Protein Components Associated with Virulence. Eukaryot Cell 7: 58-67 [Abstract] [Full Text]  
  • Akao, T., Sano, M., Yamada, O., Akeno, T., Fujii, K., Goto, K., Ohashi-Kunihiro, S., Takase, K., Yasukawa-Watanabe, M., Yamaguchi, K., Kurihara, Y., Maruyama, J.-i., Juvvadi, P. R., Tanaka, A., Hata, Y., Koyama, Y., Yamaguchi, S., Kitamoto, N., Gomi, K., Abe, K., Takeuchi, M., Kobayashi, T., Horiuchi, H., Kitamoto, K., Kashiwagi, Y., Machida, M., Akita, O. (2007). Analysis of Expressed Sequence Tags from the Fungus Aspergillus oryzae Cultured Under Different Conditions. DNA Res 0: dsm008v1-11 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»