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Applied and Environmental Microbiology, May 2006, p. 3489-3497, Vol. 72, No. 5
0099-2240/06/$08.00+0     doi:10.1128/AEM.72.5.3489-3497.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Diversity and Seasonal Dynamics of Actinobacteria Populations in Four Lakes in Northeastern Germany

Martin Allgaier and Hans-Peter Grossart*

Leibniz Institute of Freshwater Ecology and Inland Fisheries, Department of Limnology of Stratified Lakes, Alte Fischerhütte 2, D-16775 Stechlin-Neuglobsow, Germany

Received 10 January 2006/ Accepted 28 February 2006

The phylogenetic diversity and seasonal dynamics of freshwater Actinobacteria populations in four limnologically different lakes of the Mecklenburg-Brandenburg Lake District (northeastern Germany) were investigated. Fluorescence in situ hybridization was used to determine the seasonal abundances and dynamics of total Actinobacteria (probe HGC69a) and the three actinobacterial subclusters acI, acI-A, and acI-B (probes AcI-852, AcI-840-1, and AcI-840-2). Seasonal means of total Actinobacteria abundances in the epilimnia of the lakes varied from 13 to 36%, with maximum values of 30 to 58%, of all DAPI (4',6'-diamidino-2-phenylindole)-stained cells. Around 80% of total Actinobacteria belonged to the acI cluster. The two subclusters acI-A and acI-B accounted for 60 to 91% of the acI cluster and showed seasonal means of 49% (acI-B) and 23% (acI-A) in relation to the acI cluster. Total Actinobacteria and members of the clusters acI and acI-B showed distinct seasonal changes in their absolute abundances, with maxima in late spring and fall/winter. In eight clone libraries constructed from the lakes, a total of 76 actinobacterial 16S rRNA gene sequences were identified from a total of 177 clones. The majority of the Actinobacteria sequences belonged to the acI and acIV cluster. Several new clusters and subclusters were found (acSTL, scB1-4, and acIVA-D). The majority of all obtained 16S rRNA gene sequences are distinct from those of already-cultured freshwater Actinobacteria.

* Corresponding author. Mailing address: Leibniz-Institut für Gewässerökologie und Binnenfischerei, Abteilung Limnologie geschichteter Seen, Alte Fischerhütte 2, D-16775 Stechlin-Neuglobsow, Germany. Phone: 49 33082 69991. Fax: 49 33082 69917. E-mail: hgrossart{at}igb-berlin.de.

Applied and Environmental Microbiology, May 2006, p. 3489-3497, Vol. 72, No. 5
0099-2240/06/$08.00+0     doi:10.1128/AEM.72.5.3489-3497.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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