This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Peu, P.
Right arrow Articles by Dabert, P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Peu, P.
Right arrow Articles by Dabert, P.
Agricola
Right arrow Articles by Peu, P.
Right arrow Articles by Dabert, P.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, May 2006, p. 3578-3585, Vol. 72, No. 5
0099-2240/06/$08.00+0     doi:10.1128/AEM.72.5.3578-3585.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Dynamics of a Pig Slurry Microbial Community during Anaerobic Storage and Management

Pascal Peu,1 Hubert Brugère,2 Anne-Marie Pourcher,3 Monique Kérourédan,2 Jean-Jacques Godon,4 Jean-Philippe Delgenès,4 and Patrick Dabert4*

CEMAGREF, Environmental Management and Biological Treatment of Wastes Research Unit, 17 Avenue de Cucillé, CS 64427, 35044 Rennes cedex, France,1 UMR INRA/ENVT 1225 Interactions Hôtes, Agents pathogènes, Ecole nationale vétérinaire de Toulouse, 23, chemin des Capelles, 31076 Toulouse cedex, France,2 Université d'Angers, UMR MA 105 Paysages et Biodiversité, UFR Sciences, 2, Boulevard Lavoisier, 49045 Angers cedex 01, France,3 INRA, Laboratoire de Biotechnologie de l'Environnement, Avenue des Etangs, 11100 Narbonne, France4

Received 17 August 2005/ Accepted 28 February 2006

The microbial community of a pig slurry on a farm was monitored for 6 months using both molecular and cultural approaches. Sampling was carried out at all the different stages of effluent handling, from the rearing build-up to slurry spreading. Total DNA of each sample was extracted and analyzed by PCR-single-strand conformation polymorphism (SSCP) analysis using primers targeting the 16S rRNA genes from the archaeal and bacterial domains and also the Eubacterium-Clostridium, Bacillus-Streptococcus-Lactobacillus, and Bacteroides-Prevotella groups. A comparison of the SSCP profiles showed that there were rapid changes in the dominant bacterial community during the first 2 weeks of anaerobic storage and that the community was relatively stable thereafter. Several bacterial populations, identified as populations closely related to uncultured Clostridium and Porphyromonas and to Lactobacillus and Streptococcus cultured species commonly isolated from pig feces, remained present and dominant from the rearing build-up to the time of spreading. Enumeration of fecal indicators (enterococci and Escherichia coli) performed in parallel using cultural methods revealed the same trends. On the other hand, the archaeal community adapted slowly during pig slurry storage, and its diversity increased. A shift between two hydrogenotrophic methanogenic Methanobrevibacter populations from the storage pit to the pond was observed. Microorganisms present in pig slurry at the time of spreading could not be detected in soil after spreading by either molecular or cultural techniques, probably because of the detection limit inherent in the two techniques.

* Corresponding author. Mailing address: CEMAGREF, Environmental Management and Biological Treatment of Wastes Research Unit (GERE), 17 avenue de Cucillé, CS 64427, F-35044 Rennes cedex, France. Phone: 33 223 482 153. Fax: 33 223 482 115. E-mail: patrick.dabert{at}cemagref.fr.

Applied and Environmental Microbiology, May 2006, p. 3578-3585, Vol. 72, No. 5
0099-2240/06/$08.00+0     doi:10.1128/AEM.72.5.3578-3585.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Marti, R., Dabert, P., Pourcher, A.-M. (2009). Pig Manure Contamination Marker Selection Based on the Influence of Biological Treatment on the Dominant Fecal Microbial Groups. Appl. Environ. Microbiol. 75: 4967-4974 [Abstract] [Full Text]  
  • Mieszkin, S., Furet, J.-P., Corthier, G., Gourmelon, M. (2009). Estimation of Pig Fecal Contamination in a River Catchment by Real-Time PCR Using Two Pig-Specific Bacteroidales 16S rRNA Genetic Markers. Appl. Environ. Microbiol. 75: 3045-3054 [Abstract] [Full Text]  
  • Rothrock, M. J. Jr., Cook, K. L., Warren, J. G., Sistani, K. (2008). The Effect of Alum Addition on Microbial Communities in Poultry Litter. Poult. Sci. 87: 1493-1503 [Abstract] [Full Text]  
  • Duriez, P., Topp, E. (2007). Temporal Dynamics and Impact of Manure Storage on Antibiotic Resistance Patterns and Population Structure of Escherichia coli Isolates from a Commercial Swine Farm. Appl. Environ. Microbiol. 73: 5486-5493 [Abstract] [Full Text]  
  • Ufnar, J. A., Ufnar, D. F., Wang, S. Y., Ellender, R. D. (2007). Development of a Swine-Specific Fecal Pollution Marker Based on Host Differences in Methanogen mcrA Genes. Appl. Environ. Microbiol. 73: 5209-5217 [Abstract] [Full Text]  
  • Delbes, C., Ali-Mandjee, L., Montel, M.-C. (2007). Monitoring Bacterial Communities in Raw Milk and Cheese by Culture-Dependent and -Independent 16S rRNA Gene-Based Analyses. Appl. Environ. Microbiol. 73: 1882-1891 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»