This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Peu, P. Articles by Dabert, P. Search for Related Content PubMed PubMed Citation Articles by Peu, P. Articles by Dabert, P. Agricola Articles by Peu, P. Articles by Dabert, P.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, May 2006, p. 3578-3585, Vol. 72, No. 5
0099-2240/06/$08.00+0     doi:10.1128/AEM.72.5.3578-3585.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Dynamics of a Pig Slurry Microbial Community during Anaerobic Storage and Management

CEMAGREF, Environmental Management and Biological Treatment of Wastes Research Unit, 17 Avenue de Cucillé, CS 64427, 35044 Rennes cedex, France,¹ UMR INRA/ENVT 1225 Interactions Hôtes, Agents pathogènes, Ecole nationale vétérinaire de Toulouse, 23, chemin des Capelles, 31076 Toulouse cedex, France,² Université d'Angers, UMR MA 105 Paysages et Biodiversité, UFR Sciences, 2, Boulevard Lavoisier, 49045 Angers cedex 01, France,³ INRA, Laboratoire de Biotechnologie de l'Environnement, Avenue des Etangs, 11100 Narbonne, France⁴

Received 17 August 2005/ Accepted 28 February 2006

The microbial community of a pig slurry on a farm was monitored for 6 months using both molecular and cultural approaches. Sampling was carried out at all the different stages of effluent handling, from the rearing build-up to slurry spreading. Total DNA of each sample was extracted and analyzed by PCR-single-strand conformation polymorphism (SSCP) analysis using primers targeting the 16S rRNA genes from the archaeal and bacterial domains and also the Eubacterium-Clostridium, Bacillus-Streptococcus-Lactobacillus, and Bacteroides-Prevotella groups. A comparison of the SSCP profiles showed that there were rapid changes in the dominant bacterial community during the first 2 weeks of anaerobic storage and that the community was relatively stable thereafter. Several bacterial populations, identified as populations closely related to uncultured Clostridium and Porphyromonas and to Lactobacillus and Streptococcus cultured species commonly isolated from pig feces, remained present and dominant from the rearing build-up to the time of spreading. Enumeration of fecal indicators (enterococci and Escherichia coli) performed in parallel using cultural methods revealed the same trends. On the other hand, the archaeal community adapted slowly during pig slurry storage, and its diversity increased. A shift between two hydrogenotrophic methanogenic Methanobrevibacter populations from the storage pit to the pond was observed. Microorganisms present in pig slurry at the time of spreading could not be detected in soil after spreading by either molecular or cultural techniques, probably because of the detection limit inherent in the two techniques.

This article has been cited by other articles:

• Rothrock, M. J. Jr., Cook, K. L., Warren, J. G., Sistani, K. (2008). The Effect of Alum Addition on Microbial Communities in Poultry Litter. Poult. Sci. 87: 1493-1503 [Abstract] [Full Text]  
• Duriez, P., Topp, E. (2007). Temporal Dynamics and Impact of Manure Storage on Antibiotic Resistance Patterns and Population Structure of Escherichia coli Isolates from a Commercial Swine Farm. Appl. Environ. Microbiol. 73: 5486-5493 [Abstract] [Full Text]  
• Ufnar, J. A., Ufnar, D. F., Wang, S. Y., Ellender, R. D. (2007). Development of a Swine-Specific Fecal Pollution Marker Based on Host Differences in Methanogen mcrA Genes. Appl. Environ. Microbiol. 73: 5209-5217 [Abstract] [Full Text]  
• Delbes, C., Ali-Mandjee, L., Montel, M.-C. (2007). Monitoring Bacterial Communities in Raw Milk and Cheese by Culture-Dependent and -Independent 16S rRNA Gene-Based Analyses. Appl. Environ. Microbiol. 73: 1882-1891 [Abstract] [Full Text]