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Applied and Environmental Microbiology, May 2006, p. 3608-3614, Vol. 72, No. 5
0099-2240/06/$08.00+0     doi:10.1128/AEM.72.5.3608-3614.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Uranium(VI) Reduction by Anaeromyxobacter dehalogenans Strain 2CP-C

Qingzhong Wu,1 Robert A. Sanford,3 and Frank E. Löffler1,2*

School of Civil and Environmental Engineering,1 School of Biology, Georgia Institute of Technology, Atlanta, Georgia 30332-0512,2 Department of Geology, University of Illinois, Urbana, Illinois 61801-23523

Received 2 December 2005/ Accepted 16 March 2006

Previous studies demonstrated growth of Anaeromyxobacter dehalogenans strain 2CP-C with acetate or hydrogen as the electron donor and Fe(III), nitrate, nitrite, fumarate, oxygen, or ortho-substituted halophenols as electron acceptors. In this study, we explored and characterized U(VI) reduction by strain 2CP-C. Cell suspensions of fumarate-grown 2CP-C cells reduced U(VI) to U(IV). More-detailed growth studies demonstrated that hydrogen was the required electron donor for U(VI) reduction and could not be replaced by acetate. The addition of nitrate to U(VI)-reducing cultures resulted in a transitory increase in U(VI) concentration, apparently caused by the reoxidation of reduced U(IV), but U(VI) reduction resumed following the consumption of N-oxyanions. Inhibition of U(VI) reduction occurred in cultures amended with Fe(III) citrate, or citrate. In the presence of amorphous Fe(III) oxide, U(VI) reduction proceeded to completion but the U(VI) reduction rates decreased threefold compared to control cultures. Fumarate and 2-chlorophenol had no inhibitory effects on U(VI) reduction, and both electron acceptors were consumed concomitantly with U(VI). Since cocontaminants (e.g., nitrate, halogenated compounds) and bioavailable ferric iron are often encountered at uranium-impacted sites, the metabolic versatility makes Anaeromyxobacter dehalogenans a promising model organism for studying the complex interaction of multiple electron acceptors in U(VI) reduction and immobilization.

* Corresponding author. Mailing address: Georgia Institute of Technology, School of Civil and Environmental Engineering, 311 Ferst Drive, 3228 ES&T Building, Atlanta, GA 30332-0512. Phone: (404) 894-0279. Fax: (404) 894-8266. E-mail: frank.loeffler{at}ce.gatech.edu.

Applied and Environmental Microbiology, May 2006, p. 3608-3614, Vol. 72, No. 5
0099-2240/06/$08.00+0     doi:10.1128/AEM.72.5.3608-3614.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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