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Applied and Environmental Microbiology, May 2006, p. 3738-3742, Vol. 72, No. 5
0099-2240/06/$08.00+0     doi:10.1128/AEM.72.5.3738-3742.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

arsRBOCT Arsenic Resistance System Encoded by Linear Plasmid pHZ227 in Streptomyces sp. Strain FR-008

Lianrong Wang,1 Shi Chen,1,{dagger} Xiang Xiao,2 Xi Huang,2 Delin You,1 Xiufen Zhou,1,2 and Zixin Deng1,2*

Laboratory of Microbial Metabolism and School of Life Science and Biotechnology, Shanghai Jiaotong University, Shanghai 200030, China,1 School of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China2

Received 20 November 2005/ Accepted 1 February 2006

In the arsenic resistance gene cluster from the large linear plasmid pHZ227, two novel genes, arsO (for a putative flavin-binding monooxygenase) and arsT (for a putative thioredoxin reductase), were coactivated and cotranscribed with arsR1-arsB and arsC, respectively. Deletion of the ars gene cluster on pHZ227 in Streptomyces sp. strain FR-008 resulted in sensitivity to arsenic, and heterologous expression of the ars gene cluster in the arsenic-sensitive Streptomyces strains conferred resistance on the new hosts. The pHZ227 ArsB protein showed homology to the yeast arsenite transporter Acr3p. The pHZ227 ArsC appears to be a bacterial thioredoxin-dependent ArsC-type arsenate reductase with four conserved cysteine thioredoxin-requiring motifs.

* Corresponding author. Mailing address: School of Life Science and Biotechnology, Shanghai Jiaotong University, 1954 Huashan Road, Shanghai 200030, People's Republic of China. Phone: 86 21 62933404. Fax: 86 21 62932418. E-mail: zxdeng{at}sjtu.edu.cn.

{dagger} Present address: Department of Chemistry, Massachusetts Institute of Technology, Boston, Mass.

Applied and Environmental Microbiology, May 2006, p. 3738-3742, Vol. 72, No. 5
0099-2240/06/$08.00+0     doi:10.1128/AEM.72.5.3738-3742.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Wang, L., Jeon, B., Sahin, O., Zhang, Q. (2009). Identification of an Arsenic Resistance and Arsenic-Sensing System in Campylobacter jejuni. Appl. Environ. Microbiol. 75: 5064-5073 [Abstract] [Full Text]  
  • Zhang, Y., Ma, Y.-F., Qi, S.-W., Meng, B., Chaudhry, M. T., Liu, S.-Q., Liu, S.-J. (2007). Responses to arsenate stress by Comamonas sp. strain CNB-1 at genetic and proteomic levels. Microbiology 153: 3713-3721 [Abstract] [Full Text]  


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