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Applied and Environmental Microbiology, May 2006, p. 3746-3749, Vol. 72, No. 5
0099-2240/06/$08.00+0     doi:10.1128/AEM.72.5.3746-3749.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Influence of Sporulation Medium Composition on Transcription of ger Operons and the Germination Response of Spores of Bacillus cereus ATCC 14579

Luc M. Hornstra,1,2 Ynte P. de Vries,1,2,3 Willem M. de Vos,1 and Tjakko Abee1,3*

Wageningen Centre for Food Sciences, Wageningen University, Wageningen, The Netherlands,1 Agrotechnology and Food Innovations, Wageningen University, Wageningen, The Netherlands,2 Laboratory of Food Microbiology, Wageningen University, Wageningen, The Netherlands3

Received 14 November 2005/ Accepted 16 February 2006

Bacillus cereus ATCC 14579 endospores were produced in Y1 medium, a nutrient-rich, chemically defined sporulation medium, and in modified G medium, containing low amounts of nutrients. The average transcription level of the seven ger operons per cell was 3.5 times higher in Y1 medium, and the spores grown in this medium showed an enhanced germination response.


* Corresponding author. Mailing address: Laboratory of Food Microbiology, Wageningen University, Bomenweg 2, 6703 HD Wageningen, The Netherlands. Phone: 31-317-484981. Fax: 31-317-484978. E-mail: tjakko.abee{at}wur.nl.


Applied and Environmental Microbiology, May 2006, p. 3746-3749, Vol. 72, No. 5
0099-2240/06/$08.00+0     doi:10.1128/AEM.72.5.3746-3749.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.




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