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Applied and Environmental Microbiology, May 2006, p. 3756-3758, Vol. 72, No. 5
0099-2240/06/$08.00+0     doi:10.1128/AEM.72.5.3756-3758.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Involvement of Coenzyme M during Aerobic Biodegradation of Vinyl Chloride and Ethene by Pseudomonas putida Strain AJ and Ochrobactrum sp. Strain TD{dagger}

Anthony S. Danko,1 Christopher A. Saski,2 Jeffrey P. Tomkins,2 and David L. Freedman3*

Centro de Engenharia Biológica, Universidade do Minho, Campus Gualtar, Braga 4710-057, Portugal,1 Clemson University Genomics Institute, 100 Jordan Hall, Clemson University, Clemson, South Carolina 29634,2 Department of Environmental Engineering and Science, Clemson University, Clemson, South Carolina 296343

Received 7 September 2005/ Accepted 22 February 2006

The involvement of coenzyme M in aerobic biodegradation of vinyl chloride and ethene in Pseudomonas putida strain AJ and Ochrobactrum sp. strain TD was demonstrated using PCR, hybridization, and enzyme assays. The results of this study extend the range of eubacteria known to use epoxyalkane:coenzyme M transferase.

* Corresponding author. Mailing address: Department of Environmental Engineering & Science, Box 340919, Clemson University, Clemson, SC 29634-0919. Phone: (864) 656-5566. Fax: (864) 656-0672. E-mail: dfreedm{at}clemson.edu.

{dagger} Supplemental material for this article may be found at http://aem.asm.org/.

Applied and Environmental Microbiology, May 2006, p. 3756-3758, Vol. 72, No. 5
0099-2240/06/$08.00+0     doi:10.1128/AEM.72.5.3756-3758.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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