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Applied and Environmental Microbiology, May 2006, p. 3766-3770, Vol. 72, No. 5
0099-2240/06/$08.00+0     doi:10.1128/AEM.72.5.3766-3770.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Comparison of Cultures from Rectoanal-Junction Mucosal Swabs and Feces for Detection of Escherichia coli O157 in Dairy Heifers

Margaret A. Davis,1,{dagger} Daniel H. Rice,1,2,{ddagger} Haiqing Sheng,1 Dale D. Hancock,2 Thomas E. Besser,3 Rowland Cobbold,2 and Carolyn J. Hovde1*

Department of Microbiology, Molecular Biology, and Biochemistry, University of Idaho, Moscow, Idaho 83844-3052,1 Department of Veterinary Clinical Sciences, Washington State University, Pullman, Washington 99164-6610,2 Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington 99164-70403

Received 20 September 2005/ Accepted 28 February 2006

Fecal culture for Escherichia coli O157:H7 was compared to rectoanal mucosal swab (RAMS) culture in dairy heifers over a 1-year period. RAMS enrichment culture was as sensitive as fecal culture using immunomagnetic separation (IMS) (P = 0.98, as determined by a chi-square test). RAMS culture is less costly than fecal IMS culture and can yield quantitative data.

* Corresponding author. Mailing address: Department of Microbiology, Molecular Biology, and Biochemistry, University of Idaho, Moscow, ID 83844-3052. Phone: (208) 885-5906. Fax: (208) 885-6518. E-mail: cbohach{at}uidaho.edu.

{dagger} Present address: Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164-7040.

{ddagger} Present address: Food Laboratory Division, New York State Department of Agriculture and Markets, Albany, NY 12235.

Applied and Environmental Microbiology, May 2006, p. 3766-3770, Vol. 72, No. 5
0099-2240/06/$08.00+0     doi:10.1128/AEM.72.5.3766-3770.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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