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Applied and Environmental Microbiology, May 2006, p. 3780-3784, Vol. 72, No. 5
0099-2240/06/$08.00+0     doi:10.1128/AEM.72.5.3780-3784.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Development and Validation of an Oligonucleotide Microarray for Detection of Multiple Virulence and Antimicrobial Resistance Genes in Escherichia coli{dagger}

Guillaume Bruant,1 Christine Maynard,2 Sadjia Bekal,3 Isabelle Gaucher,1 Luke Masson,2 Roland Brousseau,2 and Josée Harel1*

Groupe de Recherche sur les Maladies Infectieuses du Porc, Faculté de Médecine Vétérinaire, Université de Montréal, 3200 rue Sicotte, Saint-Hyacinthe, Québec J2S 7C6,1 Biotechnology Research Institute, National Research Council of Canada, 6100 Royalmount Avenue, Montreal, Quebec H4P 2R2,2 Institut National de Santé Publique du Québec, 20045 chemin Sainte-Marie, Sainte-Anne-de-Bellevue, Québec H9X 3R5, Canada3

Received 11 October 2005/ Accepted 12 March 2006

An oligonucleotide microarray detecting 189 Escherichia coli virulence genes or markers and 30 antimicrobial resistance genes was designed and validated using DNA from known reference strains. This microarray was confirmed to be a powerful diagnostic tool for monitoring emerging E. coli pathotypes and antimicrobial resistance, as well as for environmental, epidemiological, and phylogenetic studies including the evaluation of genome plasticity.

* Corresponding author. Mailing address: Groupe de Recherche sur les Maladies Infectieuses du Porc, Faculté de Médecine Vétérinaire, Université de Montréal, 3200 rue Sicotte, Saint-Hyacinthe, Québec J2S 7C6, Canada. Phone: (450) 773-8521, ext. 8233. Fax: (450) 778-8108. E-mail: josee.harel{at}umontreal.ca.

{dagger} Supplemental material for this article may be found at http://aem.asm.org/.

Applied and Environmental Microbiology, May 2006, p. 3780-3784, Vol. 72, No. 5
0099-2240/06/$08.00+0     doi:10.1128/AEM.72.5.3780-3784.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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