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Applied and Environmental Microbiology, May 2006, p. 3793-3796, Vol. 72, No. 5
0099-2240/06/$08.00+0     doi:10.1128/AEM.72.5.3793-3796.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Combination of Multiplex PCR and PCR-Denaturing Gradient Gel Electrophoresis for Monitoring Common Sourdough-Associated Lactobacillus Species

Luca Settanni,1 Sara Valmorri,1 Douwe van Sinderen,2 Giovanna Suzzi,1 Antonello Paparella,1 and Aldo Corsetti1*

Dipartimento di Scienze degli Alimenti, Sezione di Microbiologia Agro-Alimentare ed Ambientale, Università degli Studi di Teramo, Teramo, Italy,1 Alimentary Pharmabiotic Centre and Department of Microbiology, University College Cork, Cork, Ireland2

Received 30 November 2005/ Accepted 16 March 2006

A combination of denaturing gradient gel electrophoresis (DGGE) and a previously described multiplex PCR approach was employed to detect sourdough lactobacilli. Primers specific for certain groups of Lactobacillus spp. were used to amplify fragments, which were analyzed by DGGE. DGGE profiles obtained from Lactobacillus type strains acted as standards to analyze lactobacilli from four regional Abruzzo (central Italy) sourdoughs.

* Corresponding author. Mailing address: Dipartimento di Scienze degli Alimenti, Sezione di Microbiologia Agro-Alimentare ed Ambientale, Università degli Studi di Teramo, V. C. R. Lerici 1, 64023 Mosciano Sant'Angelo, Teramo, Italy. Phone: 39 (0)861 266896. Fax: 39 (0)85 8071509. E-mail: acorsetti{at}unite.it.

Applied and Environmental Microbiology, May 2006, p. 3793-3796, Vol. 72, No. 5
0099-2240/06/$08.00+0     doi:10.1128/AEM.72.5.3793-3796.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.





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