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Applied and Environmental Microbiology, June 2006, p. 3826-3831, Vol. 72, No. 6
0099-2240/06/$08.00+0     doi:10.1128/AEM.00379-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Validation of AKACID Plus as a Room Disinfectant in the Hospital Setting

Christina Kratzer,1 Selma Tobudic,1 Ojan Assadian,2 Astrid Buxbaum,1 Wolfgang Graninger,1 and Apostolos Georgopoulos1*

Department of Internal Medicine I, Division of Infectious Diseases and Chemotherapy,1 Clinical Institute for Hygiene and Medical Microbiology, Medical University of Vienna, Währinger Gürtel 18-20, 1090 Vienna, Austria2

Received 15 February 2006/ Accepted 13 March 2006

AKACID Plus, a novel polymeric guanidine with broad antimicrobial activity against multiantibiotic-resistant bacterial strains, was used in the present study as a room disinfectant. Disinfection of closed rooms experimentally contaminated with antibiotic-susceptible and multiresistant Staphylococcus aureus (MRSA), Pseudomonas aeruginosa, and Escherichia coli was performed using AKACID Plus at concentrations of 0.1, 0.25, and 0.5% for 100 min. Bacterial suspensions were distributed on plastic and stainless steel plates and placed in a test room. Recovery of the test microorganisms was determined before nebulizing, 60 and 100 min after initiation, and 4 h after the end of room disinfection by a simple swab-rinse technique. The swab-rinse method demonstrated a dose- and time-dependent effectiveness of AKACID Plus in eradicating S. aureus, E. coli, and P. aeruginosa on plastic and stainless steel plates. Nebulized 0.5% AKACID Plus was successful in eliminating all hospital pathogens within 340 min. After the use of 0.25% AKACID Plus, MRSA was still detectable on microbial carrier plates. The test concentration of 0.1% AKACID Plus achieved a significant reduction of S. aureus and P. aeruginosa on plastic and stainless steel plates but was sufficient to eradicate only E. coli. These results suggest that nebulized AKACID Plus at a concentration of 0.5% is a potent substance for eradication of pathogenic organisms in the hospital setting.

* Corresponding author. Mailing address: Department of Internal Medicine I, Division of Infectious Diseases and Chemotherapy, Medical University of Vienna, Währinger Gürtel 18-20, 1090 Vienna, Austria. Phone: 431 40400 5139. Fax: 431 40400 5200. E-mail: apostolos.georgopoulos{at}meduniwien.ac.at.

Applied and Environmental Microbiology, June 2006, p. 3826-3831, Vol. 72, No. 6
0099-2240/06/$08.00+0     doi:10.1128/AEM.00379-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Kratzer, C., Tobudic, S., Macfelda, K., Graninger, W., Georgopoulos, A. (2007). In Vivo Activity of a Novel Polymeric Guanidine in Experimental Skin Infection with Methicillin-Resistant Staphylococcus aureus. Antimicrob. Agents Chemother. 51: 3437-3439 [Abstract] [Full Text]  


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