This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Boghenbor, K. K.
Right arrow Articles by Frey, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Boghenbor, K. K.
Right arrow Articles by Frey, J.
Agricola
Right arrow Articles by Boghenbor, K. K.
Right arrow Articles by Frey, J.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, June 2006, p. 4061-4066, Vol. 72, No. 6
0099-2240/06/$08.00+0     doi:10.1128/AEM.01996-05
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Genotyping of Human and Porcine Yersinia enterocolitica, Yersinia intermedia, and Yersinia bercovieri Strains from Switzerland by Amplified Fragment Length Polymorphism Analysis

Kathrin Kuehni Boghenbor,1 Stephen L. W. On,2 Branko Kokotovic,2 Andreas Baumgartner,3 Trudy M. Wassenaar,4 Matthias Wittwer,1 Beatrice Bissig-Choisat,5 and Joachim Frey1*

Institute of Veterinary Bacteriology, Zentrum für Zoonosen, bakterielle Tierkrankheiten und Antibiotikaresistenz (ZOBA), University of Bern, Bern, Switzerland,1 Danish Institute for Food and Veterinary Research, Copenhagen, Denmark,2 Section of Microbiology and Biotechnology, Swiss Federal Office of Public Health, Bern-Liebefeld, Switzerland,3 Molecular Microbiology and Genomics Consultants, Zotzenheim, Germany,4 Laboratories, Federal Veterinary Office, Bern-Liebefeld, Switzerland5

Received 25 August 2005/ Accepted 16 March 2006

In this study, 231 strains of Yersinia enterocolitica, 25 strains of Y. intermedia, and 10 strains of Y. bercovieri from human and porcine sources (including reference strains) were analyzed using amplified fragment length polymorphism (AFLP), a whole-genome fingerprinting method for subtyping bacterial isolates. AFLP typing distinguished the different Yersinia species examined. Representatives of Y. enterocolitica biotypes 1A, 1B, 2, 3, and 4 belonged to biotype-related AFLP clusters and were clearly distinguished from each other. Y. enterocolitica biotypes 2, 3, and 4 appeared to be more closely related to each other (83% similarity) than to biotypes 1A (11%) and 1B (47%). Biotype 1A strains exhibited the greatest genetic heterogeneity of the biotypes studied. The biotype 1A genotypes were distributed among four major clusters, each containing strains from both human and porcine sources, confirming the zoonotic potential of this organism. The AFLP technique is a valuable genotypic method for identification and typing of Y. enterocolitica and other Yersinia spp.

* Corresponding author. Mailing address: Institute of Veterinary Bacteriology, Postfach, CH-3001 Bern, Switzerland. Phone: 41 31 631 24 14. Fax: 41 31 631 26 34. E-mail: joachim.frey{at}vbi.unibe.ch.

Applied and Environmental Microbiology, June 2006, p. 4061-4066, Vol. 72, No. 6
0099-2240/06/$08.00+0     doi:10.1128/AEM.01996-05
Copyright © 2006, American Society for Microbiology. All Rights Reserved.





Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»