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Applied and Environmental Microbiology, June 2006, p. 4225-4231, Vol. 72, No. 6
0099-2240/06/$08.00+0     doi:10.1128/AEM.00150-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Dipeptide Synthesis by an Aminopeptidase from Streptomyces septatus TH-2 and Its Application to Synthesis of Biologically Active Peptides

Jiro Arima, Yoshiko Uesugi, Misugi Uraji, Masaki Iwabuchi, and Tadashi Hatanaka*

Research Institute for Biological Sciences (RIBS), Okayama, 7549-1 Kibichuo-cho, Kaga-gun, Okayama 716-1241, Japan

Received 19 January 2006/ Accepted 12 April 2006

Dipeptide synthesis by aminopeptidase from Streptomyces septatus TH-2 (SSAP) was demonstrated using free amino acid as an acyl donor and aminoacyl methyl ester as an acyl acceptor in 98% methanol (MeOH). SSAP retained its activity after more than 100 h in 98% MeOH, and in the case of phenylalanyl-phenylalanine methyl ester synthesis, the enzyme reaction reached equilibrium when more than 50% of the free phenylalanine was converted to the product. In an investigation of the specificity of SSAP toward acyl donors and acyl acceptors, SSAP showed a broad specificity toward various free amino acids and aminoacyl methyl esters. Furthermore, we applied SSAP to the synthesis of several biologically active peptides, such as aspartyl-phenylalanine, alanyl-tyrosine, and valyl-tyrosine methyl esters.

* Corresponding author. Mailing address: Research Institute for Biological Sciences (RIBS), Okayama, 7549-1 Kibichuo-cho, Kaga-gun, Okayama 716-1241, Japan. Phone: 81-866-56-9452. Fax: 81-866-56-9454. E-mail: hatanaka{at}bio-ribs.com.

Applied and Environmental Microbiology, June 2006, p. 4225-4231, Vol. 72, No. 6
0099-2240/06/$08.00+0     doi:10.1128/AEM.00150-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Steinle, A., Bergander, K., Steinbuchel, A. (2009). Metabolic Engineering of Saccharomyces cerevisiae for Production of Novel Cyanophycins with an Extended Range of Constituent Amino Acids. Appl. Environ. Microbiol. 75: 3437-3446 [Abstract] [Full Text]  
  • Arima, J., Uesugi, Y., Iwabuchi, M., Hatanaka, T. (2006). Change in Substrate Preference of Streptomyces Aminopeptidase through Modification of the Environment around the Substrate Binding Site. Appl. Environ. Microbiol. 72: 7962-7967 [Abstract] [Full Text]  


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