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Applied and Environmental Microbiology, June 2006, p. 4426-4428, Vol. 72, No. 6
0099-2240/06/$08.00+0     doi:10.1128/AEM.02809-05
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Immobilization and Detection of Listeria monocytogenes

B. D. Schindler and L. A. Shelef*

Department of Nutrition and Food Science, Wayne State University, Detroit, Michigan

Received 29 November 2005/ Accepted 20 March 2006

A procedure was developed for immobilization of Listeria monocytogenes cells on metal hydroxides coupled with detection and enumeration using an automated optical system. The results of the immobilization procedure (<1 h) and detection during overnight incubation agreed with calculated plate counts, and this technique is simple and rapid and provides samples that are ready for confirmation of the presence of the pathogen by rapid methods.

* Corresponding author. Mailing address: 3009 Science Hall, Wayne State University, Detroit, MI 48202. Phone: (313) 577-2998. Fax: (313) 577-8616. E-mail: lshelef{at}wayne.edu.

Applied and Environmental Microbiology, June 2006, p. 4426-4428, Vol. 72, No. 6
0099-2240/06/$08.00+0     doi:10.1128/AEM.02809-05
Copyright © 2006, American Society for Microbiology. All Rights Reserved.





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