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Applied and Environmental Microbiology, June 2006, p. 4429-4430, Vol. 72, No. 6
0099-2240/06/$08.00+0     doi:10.1128/AEM.02923-05

SHORT REPORT

Evaluation of a Macrofoam Swab Protocol for the Recovery of Bacillus anthracis Spores from a Steel Surface

L. R. Hodges,* L. J. Rose, A. Peterson, J. Noble-Wang, and M. J. Arduino

Centers for Disease Control and Prevention, National Center for Infectious Diseases, Division of Healthcare Quality Promotion, Atlanta, Georgia

Received 12 December 2005/ Accepted 21 March 2006

A protocol to recover Bacillus anthracis spores from a steel surface using macrofoam swabs was evaluated for its accuracy, precision, reproducibility, and limit of detection. Macrofoam swabs recovered 31.7 to 49.1% of spores from 10-cm2 steel surfaces with a ≤32.7% coefficient of variation in sampling precision and reproducibility for inocula of ≥38 spores.

* Corresponding author. Mailing address: Centers for Disease Control and Prevention, 1600 Clifton Rd., Mail Stop C16, Atlanta, GA 30333. Phone: (404) 639-4865. Fax: (404) 639-3822. E-mail: lwh9{at}cdc.gov.

Applied and Environmental Microbiology, June 2006, p. 4429-4430, Vol. 72, No. 6
0099-2240/06/$08.00+0     doi:10.1128/AEM.02923-05




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