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Applied and Environmental Microbiology, June 2006, p. 4484-4488, Vol. 72, No. 6
0099-2240/06/$08.00+0     doi:10.1128/AEM.00189-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Quantification of In Vitro and In Vivo Cryptosporidium parvum Infection by Using Real-Time PCR

Nihal T. Godiwala,1 Alain Vandewalle,2 Honorine D. Ward,1 and Brett A. Leav1*

Division of Geographic Medicine and Infectious Diseases, Tufts-New England Medical Center, Boston, Massachusetts 02111,1 INSERM, U773, Centre de Recherche Biomédicale Bichat-Beaujon (CRB3), BP 416, F-75018 Paris Université Paris 7—Denis Diderot, site Bichat, F-75870 Paris, France2

Received 24 January 2006/ Accepted 9 April 2006

Established methods for quantifying experimental Cryptosporidium infection are highly variable and subjective. We describe a new technique using quantitative real-time PCR (qPCR) that can be used to measure in vitro and in vivo laboratory infections with Cryptosporidium. We show for the first time that qPCR permits absolute quantification of the parasite while simultaneously controlling for the amount of host tissue and correlates significantly with established methods of quantification in in vitro and in vivo laboratory models of infection.

* Corresponding author. Mailing address: Division of Geographic Medicine and Infectious Diseases, Tufts-New England Medical Center, Box 041, 750 Washington Street, Boston, MA 02111. Phone: (617) 636-2869. Fax: (617) 636-5292. E-mail: bleav{at}tufts-nemc.org.

Applied and Environmental Microbiology, June 2006, p. 4484-4488, Vol. 72, No. 6
0099-2240/06/$08.00+0     doi:10.1128/AEM.00189-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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