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Applied and Environmental Microbiology, June 2006, p. 4489-4491, Vol. 72, No. 6
0099-2240/06/$08.00+0     doi:10.1128/AEM.00346-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Evaluation of the Duopath Legionella Lateral Flow Assay for Identification of Legionella pneumophila and Legionella Species Culture Isolates

Jürgen Herbert Helbig,1* Paul Christian Lück,1 Britta Kunz,2 and Andreas Bubert2

Institute of Medical Microbiology and Hygiene, Medical Faculty of the Technical University Dresden, Dresden,1 Life Science & Analytics Division, Merck KGaA, Darmstadt, Germany2

Received 13 February 2006/ Accepted 10 April 2006

Duopath Legionella (Merck KGaA, Darmstadt, Germany) is a new immunochromatographic assay for the simultaneous identification of cultured L. pneumophila and Legionella species other than L. pneumophila. In tests of 89 L. pneumophila strains and 87 Legionella strains other than L. pneumophila representing 41 different species, Duopath and a widely used latex agglutination assay detected L. pneumophila with 100% and 98% accuracy, respectively, whereas the percentages differed significantly for other Legionella spp. (93% versus 37% [P < 0.001]). Since many countries’ regulations require the identification of Legionella spp. in water and environmental samples, the use of Duopath Legionella to comply with those regulations could contribute to significantly fewer false-negative results.

* Corresponding author. Mailing address: Institute of Medical Microbiology and Hygiene, Medical Faculty of the Technical University Dresden, Fetscherstr. 74, D-01307 Dresden, Germany. Phone: 49-351-4586577. Fax: 49-351-4586310. E-mail: j.helbig{at}mailbox.tu-dresden.de.

Applied and Environmental Microbiology, June 2006, p. 4489-4491, Vol. 72, No. 6
0099-2240/06/$08.00+0     doi:10.1128/AEM.00346-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.





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