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Applied and Environmental Microbiology, July 2006, p. 4604-4609, Vol. 72, No. 7
0099-2240/06/$08.00+0     doi:10.1128/AEM.00081-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Biodesulfurization in Biphasic Systems Containing Organic Solvents{dagger}

Fei Tao,{ddagger} Bo Yu,{ddagger} Ping Xu,* and Cui Qing Ma

State Key Lab of Microbial Technology, Shandong University, Jinan 250100, People's Republic of China

Received 12 January 2006/ Accepted 25 April 2006

Biphasic systems can overcome the problem of low productivity in conventional media and have been exploited for biocatalysis. Solvent-tolerant microorganisms are useful in biotransformation with whole cells in biphasic reactions. A solvent-tolerant desulfurizing bacterium, Pseudomonas putida A4, was constructed by introducing the biodesulfurizing gene cluster dszABCD, which was from Rhodococcus erythropolis XP, into the solvent-tolerant strain P. putida Idaho. Biphasic reactions were performed to investigate the desulfurization of various sulfur-containing heterocyclic compounds in the presence of various organic solvents. P. putida A4 had the same substrate range as R. erythropolis XP and could degrade dibenzothiophene at a specific rate of 1.29 mM g (dry weight) of cells–1 h–1 for the first 2 h in the presence of 10% (vol/vol) p-xylene. P. putida A4 was also able to degrade dibenzothiophene in the presence of many other organic solvents at a concentration of 10% (vol/vol). This study is a significant step in the exploration of the biotechnological potential of novel biocatalysts for developing an efficient biodesulfurization process in biphasic reaction mixtures containing toxic organic solvents.

* Corresponding author. Mailing address: State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, People's Republic of China. Phone: 86 531 88564003. Fax: 86 531 88567250. E-mail: pingxu{at}sdu.edu.cn.

{dagger} Supplemental material for this article may be found at http://aem.asm.org/.

{ddagger} F.T. and B.Y. contributed equally to this work.

Applied and Environmental Microbiology, July 2006, p. 4604-4609, Vol. 72, No. 7
0099-2240/06/$08.00+0     doi:10.1128/AEM.00081-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

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