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Applied and Environmental Microbiology, July 2006, p. 4761-4766, Vol. 72, No. 7
0099-2240/06/$08.00+0     doi:10.1128/AEM.00436-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Bacteriocin T8, a Novel Class IIa sec-Dependent Bacteriocin Produced by Enterococcus faecium T8, Isolated from Vaginal Secretions of Children Infected with Human Immunodeficiency Virus

M. De Kwaadsteniet, T. Fraser, C. A. Van Reenen, and L. M. T. Dicks*

Department of Microbiology, Stellenbosch University, Stellenbosch, South Africa

Received 23 February 2006/ Accepted 30 April 2006

Enterococcus faecium T8, isolated from vaginal secretions of children with human immunodeficiency virus, produces a class IIa sec-dependent bacteriocin that is structurally different from three other class IIa sec-dependent bacteriocins, i.e., enterocin P and an enterocin P-like bacteriocin, produced by Enterococcus faecium, and bacteriocin 31, produced by Enterococcus faecalis, and from a class III bacteriocin produced by E. faecalis. The genes encoding the bacteriocin, immunity protein, mobilization protein, and relaxase nuclease are located on a 7-kb plasmid. Bacteriocin T8 has a molecular mass of 5.1 kDa based on its DNA sequence, similar to the 5.0 kDa recorded for bacteriocin 31 but larger than the 4.6 kDa reported for enterocin P. At the amino acid level, bacteriocin T8 is 69% homologous to bacteriocin 31 and 47% homologous to enterocin P. Bacteriocin T8 is active against E. faecalis isolated from patients diagnosed with vaginosis, against Lactobacillus sakei, and against a Propionibacterium sp. The peptide is heat stable (60 min at 100°C) and remains active in phosphate buffer from pH 4.0 to 10.0. The mode of activity is bactericidal, as determined with E. faecalis.

* Corresponding author. Mailing address: Department of Microbiology, Stellenbosch University, Private Bag X1, Matieland 7602, Stellenbosch, South Africa. Phone: 27-21-808 5849. Fax: 27-21-808 5846. E-mail: lmtd{at}sun.ac.za.

Applied and Environmental Microbiology, July 2006, p. 4761-4766, Vol. 72, No. 7
0099-2240/06/$08.00+0     doi:10.1128/AEM.00436-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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