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Applied and Environmental Microbiology, July 2006, p. 5104-5107, Vol. 72, No. 7
0099-2240/06/$08.00+0     doi:10.1128/AEM.02626-05
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Enhanced Survival of GroESL-Overproducing Lactobacillus paracasei NFBC 338 under Stressful Conditions Induced by Drying

B. M. Corcoran,1,2 R. P. Ross,1,3 G. F. Fitzgerald,2,3 P. Dockery,4 and C. Stanton1,3*

Teagasc, Moorepark Food Research Centre, Fermoy, County Cork, Ireland,1 Department of Microbiology, University College, Cork, Ireland,2 Alimentary Pharmabiotic Centre, Cork, Ireland,3 Department of Anatomy, University College, Cork, Ireland4

Received 7 November 2005/ Accepted 8 April 2006

GroESL-overproducing Lactobacillus paracasei NFBC 338 was dried, and its viability was compared with that of controls. Spray- and freeze-dried cultures overproducing GroESL exhibited ~10-fold and 2-fold better survival, respectively, demonstrating the importance of GroESL in stress tolerance, which can be exploited to enhance the technological performance of sensitive probiotic cultures.

* Corresponding author. Mailing address: Teagasc, Moorepark Food Research Centre, Fermoy, Co. Cork, Ireland. Phone: 353-25-42606. Fax: 353-25-42340. E-mail: cstanton{at}moorepark.teagasc.ie.

Applied and Environmental Microbiology, July 2006, p. 5104-5107, Vol. 72, No. 7
0099-2240/06/$08.00+0     doi:10.1128/AEM.02626-05
Copyright © 2006, American Society for Microbiology. All Rights Reserved.





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