This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Sachelaru, P.
Right arrow Articles by Brandsch, R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sachelaru, P.
Right arrow Articles by Brandsch, R.
Agricola
Right arrow Articles by Sachelaru, P.
Right arrow Articles by Brandsch, R.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, July 2006, p. 5126-5131, Vol. 72, No. 7
0099-2240/06/$08.00+0     doi:10.1128/AEM.00437-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

A Functional mobA Gene for Molybdopterin Cytosine Dinucleotide Cofactor Biosynthesis Is Required for Activity and Holoenzyme Assembly of the Heterotrimeric Nicotine Dehydrogenases of Arthrobacter nicotinovorans

Paula Sachelaru,1 Emile Schiltz,2 and Roderich Brandsch1*

Institute of Biochemistry and Molecular Biology,1 Institute of Organic Chemistry and Biochemistry, University of Freiburg, Freiburg, Germany2

Received 23 February 2006/ Accepted 4 May 2006

Two Arthrobacter nicotinovorans molybdenum enzymes hydroxylate the pyridine ring of nicotine. Molybdopterin cytosine dinucleotide (MCD) was determined to be a cofactor of these enzymes. A mobA gene responsible for the formation of MCD could be identified and its function shown to be required for assembly of the heterotrimeric molybdenum enzymes.

* Corresponding author. Mailing address: Institute for Biochemistry and Molecular Biology, University of Freiburg, Hermann-Herder-Strasse 7, 79104 Freiburg, Germany. Phone: 49-761-2035231. Fax: 49-761-2035253. E-mail: roderich.brandsch{at}biochemie.uni-freiburg.de.

Applied and Environmental Microbiology, July 2006, p. 5126-5131, Vol. 72, No. 7
0099-2240/06/$08.00+0     doi:10.1128/AEM.00437-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Neumann, M., Mittelstadt, G., Seduk, F., Iobbi-Nivol, C., Leimkuhler, S. (2009). MocA Is a Specific Cytidylyltransferase Involved in Molybdopterin Cytosine Dinucleotide Biosynthesis in Escherichia coli. J. Biol. Chem. 284: 21891-21898 [Abstract] [Full Text]  
  • Ganas, P., Brandsch, R. (2009). Uptake of L-nicotine and of 6-hydroxy-L-nicotine by Arthrobacter nicotinovorans and by Escherichia coli is mediated by facilitated diffusion and not by passive diffusion or active transport. Microbiology 155: 1866-1877 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»