This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lahtinen, S. J.
Right arrow Articles by Salminen, S. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lahtinen, S. J.
Right arrow Articles by Salminen, S. J.
Agricola
Right arrow Articles by Lahtinen, S. J.
Right arrow Articles by Salminen, S. J.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, July 2006, p. 5132-5134, Vol. 72, No. 7
0099-2240/06/$08.00+0     doi:10.1128/AEM.02897-05
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Intrinsic Properties of So-Called Dormant Probiotic Bacteria, Determined by Flow Cytometric Viability Assays

Sampo J. Lahtinen,1,2* Arthur C. Ouwehand,1 Johanna P. Reinikainen,2 Jaakko M. Korpela,2 Jouko Sandholm,3 and Seppo J. Salminen1,2

Department of Biochemistry and Food Chemistry, University of Turku, 20014 Turku, Finland,1 Functional Foods Forum, University of Turku, 20014 Turku, Finland,2 Cell Imaging Core, Turku Centre for Biotechnology, University of Turku and Åbo Akademi University, 20521 Turku, Finland3

Received 8 December 2005/ Accepted 9 May 2006

Plate counting and four culture-independent flow cytometric assays were used to determine the viability and intrinsic properties of three probiotic strains during storage. The strains showed reduction in plate counts but were able to maintain esterase activity, intact cytoplasmic membrane, and pH gradient. The apparently uncultivable probiotic cells were active and stress resistant.

* Corresponding author. Mailing address: Functional Foods Forum, University of Turku, Itäinen pitkäkatu 4, 20014 Turku, Finland. Phone: 358 (2) 3336861. Fax: 358 (2) 3336862. E-mail: sajola{at}utu.fi.

Applied and Environmental Microbiology, July 2006, p. 5132-5134, Vol. 72, No. 7
0099-2240/06/$08.00+0     doi:10.1128/AEM.02897-05
Copyright © 2006, American Society for Microbiology. All Rights Reserved.





Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»