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Applied and Environmental Microbiology, August 2006, p. 5283-5288, Vol. 72, No. 8
0099-2240/06/$08.00+0     doi:10.1128/AEM.00808-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Unlocking Streptomyces spp. for Use as Sustainable Industrial Production Platforms by Morphological Engineering{dagger}

Gilles P. van Wezel,1* Preben Krabben,2 Bjørn A. Traag,1 Bart J. F. Keijser,3 Rob Kerste,2 Erik Vijgenboom,1 Josef J. Heijnen,2 and Barend Kraal1

Microbial Development, LIC, Leiden University, P.O. Box 9502, 2300RA Leiden, The Netherlands,1 Department of Biotechnology, Technical University of Delft, Julianalaan 67, Delft, The Netherlands,2 TNO Voeding, Postbus 360, 3700 AJ Zeist, The Netherlands3

Received 6 April 2006/ Accepted 30 May 2006

Filamentous actinomycetes are commercially widely used as producers of natural products (in particular antibiotics) and of industrial enzymes. However, the mycelial lifestyle of actinomycetes, resulting in highly viscous broths and unfavorable pellet formation, has been a major bottleneck in their commercialization. Here we describe the successful morphological engineering of industrially important streptomycetes through controlled expression of the morphogene ssgA. This led to improved growth of many industrial and reference streptomycetes, with fragmentation of the mycelial clumps resulting in significantly enhanced growth rates in batch fermentations of Streptomyces coelicolor and Streptomyces lividans. Product formation was also stimulated, with a twofold increase in yield of enzyme production by S. lividans. We anticipate that the use of the presented methodology will make actinomycetes significantly more attractive as industrial and sustainable production hosts.

* Corresponding author. Mailing address: Microbial Development, LIC, Leiden University, P.O. Box 9502, 2300RA Leiden, The Netherlands. Phone: 31 71 5274310. Fax: 31 71 5274340. E-mail: g.wezel{at}chem.leidenuniv.nl.

{dagger} Supplemental material for this article may be found at http://aem.asm.org/.

Applied and Environmental Microbiology, August 2006, p. 5283-5288, Vol. 72, No. 8
0099-2240/06/$08.00+0     doi:10.1128/AEM.00808-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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