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Applied and Environmental Microbiology, August 2006, p. 5297-5303, Vol. 72, No. 8
0099-2240/06/$08.00+0     doi:10.1128/AEM.00464-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Occurrence of Cryptosporidium Oocysts and Giardia Cysts in Sewage in Norway

L. J. Robertson,^* L. Hermansen, and B. K. Gjerde

Parasitology Laboratory, Department of Food Safety and Infection Biology, Norwegian School of Veterinary Science, 0033 Oslo, Norway

Received 27 February 2006/ Accepted 19 May 2006

Samples of sewage influent from 40 sewage treatment works (STW) throughout Norway were examined for Cryptosporidium oocysts and Giardia duodenalis cysts. Both parasites were detected frequently (80% of STW were Cryptosporidium positive; 93% of STW were Giardia positive) and at maximum concentrations of >20,000 parasites/liter. The data suggest giardiasis is more widespread, and/or occurs with greater infection intensity, than cryptosporidiosis in Norway. STW serving higher person equivalents were more likely to be positive and had higher parasite concentrations. Parasite concentrations were used to estimate the proportion of contributing populations that could be clinically infected. For Cryptosporidium, the highest estimates were up to 5 per 100,000 individuals for two populations in eastern Norway. For Giardia, the highest estimate was 40 infected per 100,000 persons (approximately five times the usual national annual average) contributing to an STW in western Norway. As this population experienced a large waterborne giardiasis outbreak 6 months after sampling, it can be speculated that regular challenge with Giardia may occur here. Most Giardia isolates in sewage influent were assemblage A, although some assemblage B isolates were detected. There was substantial heterogeneity, but most samples contained isolates similar to genotype A3. Removal efficiencies at two STW with secondary treatment processes were estimated to be approximately 50% for Cryptosporidium and >80% for Giardia. An STW with minimal treatment had negligible removal of both parasites. Many STW in Norway have minimal treatment and discharge effluent into rivers and lakes, thus, risk of contamination of water courses by Cryptosporidium and Giardia is considerable.

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Present address: Immunology Laboratory, Department of Food Safety and Infection Biology, Norwegian School of Veterinary Science, 0033 Oslo, Norway.